Determination of limonin in rat plasma by liquid chromatography-electrospray mass spectrometry |
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Authors: | Liang Yan Xie Lin Liu Xiao-Dong Lu Tong Wang Guang-Ji Hu Yu-Zhu |
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Affiliation: | China Pharmaceutical University, Shennong Road No. 1, Nanjning 210038, China |
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Abstract: | A simple, sensitive and selective liquid chromatography coupled with electrospray ionization mass spectrometry (LC/ESI/MS) method for determination of limonin (LM) in rat plasma has been developed and validated. The method had advantages of a single liquid–liquid extraction with ether and high sensitivity. Analyses were conducted at a flow rate of 0.2 ml/min by a gradient elution. The detection utilized selected ion monitoring in the negative ion mode at m/z 460.00 and 423.15 for the deprotonated molecular ions of LM and the internal standard, respectively. The quantitation limit for LM in rat plasma was 1.0 ng/ml. The linearity was also excellent over the concentration range of 1.9–500 ng/ml of LM. The intra- and inter-day precision (relative standard deviation (R.S.D.%)) was lower than 10% and accuracy ranged from 90 to 110%, showing a good reproducibility. This developed method was successfully applied to analysis of LM in biological fluids. |
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Keywords: | Limonin LC/ESI/MS Rat plasma |
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