| 副溶血性弧菌脉冲场凝胶电泳分子分型研究 |
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| 引用本文： | 杨丽华,陈敏,陈洪友,王小光,徐彩萍. 副溶血性弧菌脉冲场凝胶电泳分子分型研究[J]. 疾病控制杂志, 2010, 14(6): 528-531 |
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| 作者姓名： | 杨丽华 陈敏 陈洪友 王小光 徐彩萍 |
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| 作者单位： | 1. 上海市闵行区疾病预防控制中心,上海,201101
2. 上海市疾病预防控制中心,上海,200336 |
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| 基金项目： | 上海市闵行区自然科学研究课题 |
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| 摘 要： | 目的用脉冲场凝胶电泳方法绘制副溶血性弧菌食物中毒分离株的DNA指纹图谱,分析各菌株间的同源性关系。方法 55株食物中毒来源的副溶血性弧菌基因组DNA经限制性内切酶NotI酶切,用脉冲场凝胶电泳方法获得电泳图谱,利用BioNumerics软件对图谱进行聚类分析。结果实验得到55株副溶血性弧菌的DNA指纹图谱,可大致分为30种PFGE图谱。聚类分析显示,在大约90%的相似性水平上,有38株菌的PFGE图谱属于同一个克隆群,为2007-2008年引起闵行区食物中毒的优势流行菌型。结论闵行地区存在遗传谱系密切相关的副溶血性弧菌流行克隆。脉冲场凝胶电泳方法是分析副溶血性弧菌同源性的有效方法 ,有助于追溯传染源。
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| 关 键 词： | 副溶血性弧菌 食物中毒 脉冲场凝胶电泳 |
Molecular typing on Vibrio parahaemolyticus strains by PFGE |
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| YANG Li-hua,CHEN Min,CHEN Hong-you,WANG Xiao-guang,XU Cai-ping. Molecular typing on Vibrio parahaemolyticus strains by PFGE[J]. Chinese Journal of Disease Control and Prevention, 2010, 14(6): 528-531 |
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| Authors: | YANG Li-hua CHEN Min CHEN Hong-you WANG Xiao-guang XU Cai-ping |
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| Affiliation: | 1.Minhang District Center for Disease Control and Prevention of Shanghai, Shanghai 201101, China; 2.Shanghai Center for Disease Control and Prevention, Shanghai 200336, China ) |
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| Abstract: | Objective To draw a DNA fingerprint of Vibrio parahaemolyticus strains by PFGE, and to determine relationship among the Vibrio parahaemolyticus isolated from food poisoning. Methods Chromosomal DNAs from 55 strains were cut by restriction enzyme Not I, and then the molecular subtying were analyzed by PFGE. The source of these strains were analyzed using software-BioNumerics. Results There were 30 distractive PFGE patterns. 38 stains belonged to the same cluster, these strains seemed to be prevailing in Minhang in two years. Conclusions There were closely related pandemic clones of Vibrio parahaemolyticus in Minhang. PFGE was very useful method in analyzing homology of Vibrio parahaemolyticus. |
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| Keywords: | Vibrio parahaemolyticus Food-poisoning Pulse-field gel electrophoresis |
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