| 核因子-κB p50亚基同源结构域相互作用多肽的筛选 |
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| 引用本文： | 沈利群,梁华平,徐祥,吕凤林,李生茂. 核因子-κB p50亚基同源结构域相互作用多肽的筛选[J]. 实用医药杂志(山东), 2006, 23(8): 949-952 |
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| 作者姓名： | 沈利群 梁华平 徐祥 吕凤林 李生茂 |
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| 作者单位： | [1]第三军医大学大坪医院野战外科研究所,重庆400042 [2]153医院,河南郑州450042 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;重庆市科委资助项目 |
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| 摘 要： | 目的应用酵母双杂交技术筛选获得与NF-kB p50亚基同源结构域(RHD)相互作用的多肽。方法应用酵母双杂交技术,以NF-kB p50 RHD为诱饵,筛选16肽cDNA文库,寻找与其相互作用的多肽,应用β-GAL实验、酵母交配实验及一对一酵母回复性杂交等方法筛除假阳性克隆,确定阳性克隆。结果经酵母双杂交及反复的排查,共获得8个阳性克隆。通过GenBank进行搜寻,未发现与之相匹配的蛋白序列。结论获得8个与NF-kB p50 RHD相互作用的新型多肽cDNA,多肽的功能需进一步验证。
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| 关 键 词： | 核因子-kB p50 酵母双杂交系统 自主报告基因活性 |
| 修稿时间： | 2006-05-08 |
Screening of polypeptides interacting with Rel homology domain of NF-κB p50 subunit |
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| SHEN Li-qun,LIANG Hua-ping,XU Xiang,et al.. Screening of polypeptides interacting with Rel homology domain of NF-κB p50 subunit[J]. Practical Journal of Medicine & Pharmacy, 2006, 23(8): 949-952 |
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| Authors: | SHEN Li-qun LIANG Hua-ping XU Xiang et al. |
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| Affiliation: | SHEN Li-qun,LIANG Hua-ping,XU Xiang,et al. Research Institute of Field Surgery,Daping Hospital,Third Military Medical University,Chongqing 400042,China |
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| Abstract: | Objective To obtain polypepddes interacting with NF-kB p50 Rel homology domain(RHD) by yeast two-hybrid technique.Methods Using NF-kB p50 RHD as bait,a 16-peptide cDNA library was screened and the polypeptides interacting with NF-kB p50 RHD were searched.The false positive clones were discarded byβ-GAL assay, yeast mating, and one to one yeast two- hybrid methods. Results 8 truly positive clones were obtained. The gene sequences of 8 polypeptides were different with each other and their homologous proteins were not found from GenBank.Conclusion 8 new polypeptides cDNAs have been obtained and their functions need to be validated in the future. |
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| Keywords: | p50 |
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