NB-UVB对HaCaT细胞CCL22表达的影响

目的：明确NB-UVB对HaCaT细胞CCL22的影响。方法：常规培养后的HaCaT细胞分为两组，一组给予不同剂量NB-UVB (0、100、200、400 mJ/cm2)照射；一组给予NF-κB抑制剂PDTC（1、12.5、25 μmol/L）预处理后再进行NB-UVB照射，采用Real-time PCR及ELISA检测CCL22表达；采用Western blot方法检测NF-κB p65磷酸化水平。结果：CCL22表达和NF-κB p65磷酸化的水平随着NB-UVB照射剂量增强而上调；PDTC处理后，NB-UVB诱导的角质形成细胞CCL22表达及和NF-κB p65磷酸化水平较直接给予NB-UVB明显下调，且PDTC浓度越高越明显。 结论：NB-UVB照射可能通过激活NF-κB信号通路促进角质形成细胞CCL22表达及分泌。

Effects of NB-UVB on the expression of CCL22 in HaCaT cells

Objective: To determine the effects of NB-UVB on the expression of CCL22 from HaCaT cells. Methods: The cultrued HaCaT cells were divided into two groups. The HaCaT cells in one group were treated with different dose of NB-UVB (0,100,200,400 mJ/ cm2 ) and those in the second group were treated with NB-UVB (400 mJ/ cm2 ) after the pretreatment with PDTC. The expression of CCL22 was detected by Real-time PCR and ELISA. The level of NF-κB p65 phosphorylation was detected by western blot. Results: The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells treated with NB-UVB were increased as the dose of of NB-UVB was increasing. The expression of CCL22 and the level of NF-κB p65 phosphorylation in HaCaT cells pretreated by PDTC were lower than those treated with NB-UVB only and the higher the concentration of PDTC, the effects were more obvious. Conclusion: The CCL22 expression induced by NB-UVB in keratinocytes may through activation of NF-κB pathway.