朗格汉斯细胞表面分子与尖锐湿疣复发的相关性

目的:确定朗格汉斯细胞(Langerhans cells,LC)表面分子与尖锐湿疣(condyloma acuminatum,CA)复发的相关性。方法:采用免疫组化方法对正常包皮、CA初发和复发患者皮损组织中LC进行CD1a、S100、HLA-DR标记,并对其表达强度进行半定量分析。结果:正常对照组、CA初发组及CA复发组CD1a免疫组化染色IOD值分别为7644.84±2865.10、4075.62±2410.39和2308.08±1256.34;S100分别为4400.34±2962.29、1815.78±1013.35及1414.46±1337.13,CA组织中LC的CD1a及S100分子表达较正常组织显著下降(P0.05),在复发组尤为明显。HLA-DR IOD值分别为583.91±592.85、2262.87±2983.09和1191.75±1698.36。HLA-DR的表达在CA初发组和复发组均强于正常组,但复发组较初发组下降。结论:HPV感染人体后,会导致HPV感染的免疫逃避,造成CA的持续感染和易复发。

Relationship between the surface molecules of Langerhans cells and recurrence of condyloma acumina-tum

Objective: To determine the relationship between the surface molecules of langerhans cells ( LC) and recurrence of condyloma acuminatum ( CA) . Methods:The expression of CD1a, S100 and HLA-DR of LC was measured by immunohistochemical technique, and their semi-quantities were analyzed by graphic software in skin samples of control group, primary CA group and recurrent CA group. Results:In con-trol group, primary CA group and recurrent CA group, the IOD of immunohistochemical staining of CD1a were 7644.84±2865.10, 4075.62±2410.39 and 2308.08±1256.34; The IOD of S100 were 4400.34±2962.29, 1815.78±1013.35 and 1414.46±1337.13. The staining intensity of CD1a and S100 in CA decreased signifi-cantly when compared with control group ( P<0.05) , with an even more decrease in the recurrent CA group. The IOD of HLA-DR were 583.91±592.85, 2262.87±2983.09 and 1191.75±1698.36 in the three groups re-spectively. The staining intensity of HLA-DR was increased in primary and recurrent CA groups, when com-pared with control group, but the expression in recurrent group decreased when compared with primary group. Conclusion: After infection of HPV, immune escape will occure and lead to the persistence and recurrence of CA.