功能失调性子宫出血和细胞凋亡,bcl-2及bax基因表达的关系

目的研究功能失调性子宫出血与细胞凋亡及其相关基因表达产物的关系。方法应用TUNEL技术检测30例正常月经周期的增生期子宫内膜，28例简单增生，2例复杂增生子宫内膜标本的凋亡指数（AI）。应用免疫组化方法检测bcl-2及bax基因在子宫内膜的表达。结果简单增生和复杂增生子宫内膜AI比正常增生期AI高（P＜0．05）；bcl-2基因在正常增生期表达比在简单增生和复杂增生表达略高，差异无显著性（P＞0．05）；bax基因在简单增生和复杂增生表达比在正常增生期表达高（P＜0．05）。结论功能失调性子宫出血的发病与细胞凋亡有关，且与bcl-2，bax表达相关。

Relationship between dysfunctional uterine bleeding and apoptosis of endometrial cells, expression of bcl-2 and bax

Objective To investigate the relationship between dysfunctional uterine bleeding and apoptosis of endometrial cells, expression of regulatory genes. Methods Human endometrial tissues were obtained from 30 patients with simple and complex hyperplasia, and from 30 controls with normal menstruation. Apoptotic index (AI) were detected using in situ Tdt-mediated dUTP-biotin nick ending labeling (TUNEL). Bcl-2 and bax were examined immunohistochemically. Rusults AI in simple and complex hyperplasia were significantly higher than that in normal proliferative phase (P<0.05). The staining of bcl-2 in cytoplasm of glandular cells was mainly seen in normal proliferation phase, and were also seen in simple and complex hyperplasia (P>0.05). Bax was significantly more in simple and complex hyperplasia than that in normal proliferation phase (P<0.05). Conclusion The abnormal endometrial cell apoptosis may play a role in the dysfunctional uterine bleeding, which is associated with bcl-2, bax expression.