胃腺癌组织中肿瘤坏死因子相关凋亡诱导配体受体-4的表达

目的:探讨肿瘤坏死因子相关凋亡诱导配体-4(TRAIL-R4)在诱导胃癌细胞凋亡过程中的作用.方法:应用Western blot、RT-PCR、原位杂交和免疫组织化学技术,检测31例正常胃黏膜和胃腺癌组织中TRAIL-附蛋白和mRNA的表达.结果:Western blot结果显示,正常胃黏膜组织中TRAIL-R4蛋白的阳性表达率80.65%,高于其在胃腺癌组织中的阳性表达率6.45%(P＜0.05);免疫组织化学结果显示,正常胃黏膜组织中TRAIL-R4蛋白均有强染色,在胃腺癌组织中染色均为阴性(P＜0.05);RT-PCR结果显示,正常胃黏膜组织中TRAIL-R4 mRNA表达高于胃腺癌组织中的表达(P＜0.05);原位杂交结果显示,正常胃黏膜组织中TRAIL-R4 mRNA染色均为阳性,而在胃腺癌组织中阳性表达率为3.23%(P＜0.05).结论:TRAIL-R4在胃癌组织中的表达缺失是TRAIL特异性诱导肿瘤细胞凋亡的原因之一.

Expression of tumor necrosis factor related apoptosis inducing ligand receptor-4 in gastric adenocarcinoma tissue

Aim: To investigate the mechanism of tumor necrosis factor related apoptosis inducing ligand receptor 4 (TRAIL R4)in the apoptosis of gastric carcinoma cell. Methods: The expression of TRAIL R4 in 31 cases of normal gastric mucosa and 31 cases of gastric adenocarcinoma tissue was detected using PT PCR, Western blot, in situ hybridization,and immunohistochemistry. Results: The positive rate of TRAIL -R4 protein in normal mucosa(80.65%) was significantly higher than that in gastric adenocarcinoma(6.45%)by Western blot(P<0.05). The expression of TRAIL R4 protein was not examined in the gastric adenocarcinoma but expressed highly in normal mucosa (100%)by using the technology of immunohistochemistry SP (P<0.05). The expression of TRAIL R4 mRNA level in normal mucosa was significantly higher than that in gastric adenocarcinoma by RT-G PCR(P<0.05).The results of TRAIL R4 mRNA by using in situ hybridization showed that the positive rates were 100% and 3.23% in normal gastric mucosa and gastric adenocarcinoma tissue, respectively(P<0.05). Conclusion: The deficiency expression of TRAIL -4 in gastric carcinoma might involve in the apoptosis of tumor cells induced by TRAIL.