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| 新生大鼠支气管上皮衍生舒张因子信号传导 | |
| 引用本文： | 刘炀,刘国津,Mitchell P Fink. 新生大鼠支气管上皮衍生舒张因子信号传导[J]. 中国实验诊断学, 2009, 13(5): 581-583 |
| 作者姓名： | 刘炀 刘国津 Mitchell P Fink |
| 作者单位： | 1. 吉林大学第一医院,胸外科,吉林,长春,130012;美国匹兹堡大学,重症监护医学实验室;吉林省肿瘤医院,胸一科 2. 吉林大学第一医院,胸外科,吉林,长春,130012;美国匹兹堡大学,重症监护医学实验室 3. 美国匹兹堡大学,重症监护医学实验室 |
| 摘 要： | 目的研究支气管上皮衍生舒张因子（BrEpDRF）是否经一氧化氮（NO）-3＇，5＇-鸟嘌呤核苷环一磷酸（cGMP）及磷脂酰三肌醇（PI3）／蛋白激酶B（Akt）通路传导。方法支气管结合的肺动脉经血栓烷A2（TXA2）类似物U46619预刺激肺动脉收缩，在PI-3激酶／Akt抑制剂LY294002和NO-GMP抑制剂ODQ存在／不存在下，测定乙酰甲胆碱（Mch）引起的血管舒张比率。结果结合支气管的肺动脉在经U46619预刺激后，Mch引起血管舒张率为39．37％；Mch中加入PI-3激酶／Akt抑制剂引起的血管舒张率为11．72％，两者比较差异有显著性，（P〈0．01）。Mch中加入NO-GMP抑制剂引起的血管舒张率为2．15％，与单一Mch比较统计学上有差异，（P〈0．01）。结论新生大鼠在生理条件下BrEpDRF经NO-cGMP及PI3／Akt通路传导。 |
| 关 键 词： | 支气管支气管上皮衍生舒张因子 肺血管阻力 一氧化氮 一氧化氮合成酶 磷脂酰肌醇-3激酶/蛋白激 酶B途径 3',5'-鸟嘌呤核苷环一磷酸 |
Bronchial epithelium derived relaxing factor signal transduction of neonate rat |
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| LIU Yang,LIU Guo-jin,Mitchell P Fink. Bronchial epithelium derived relaxing factor signal transduction of neonate rat[J]. Chinese Journal of Laboratory Diagnosis, 2009, 13(5): 581-583 | |
| Authors: | LIU Yang LIU Guo-jin Mitchell P Fink |
| Affiliation: | LIU Yang , LIU Guo-jin , FINK Mitchell P. ( 1. Department of Thoracic Surgery, First hospital, Jilin University, Changchun, 130021, China ;2. Critica/Care Medic/he Lab, University of Pittsburgh, Pittsburgh, PA, 15213; USA;3. Department of Thoracic Surgery, Jilin Province Tumor Hospital, Changchun, 130012, China) |
| Abstract: | Objective To research if bronchial epithelium derived relaxing factor （BrEpDRF） is transducted by PI3/Akt and NO-eGMP pathway.Methods Observe vessel relaxation rate of pulmonary artery with bronchus attached stimulated by methacholine （Mch） under/not under PI3/Akt inhibitor LY294002 or NO-GMP inhibitor ODQ after pre stimulated by U46619.Results After stimulated by U46619,the relax rate of pulmonary artery with bronchus attached is 39.37% stimulated by Mch,and the relax rate is 11.72 % stimulated by Meh with PI3/Akt inhibitor LY294002, compared significantly （ P 〈 0.01 ） ;the relax rate is 2.15 % stimulated by Mch with NO-GMP inhibitor ODQ, which is less than that of stimulated by Meh alone statistically （ P 〈 0.01） ,respectively. Conclusion Under physiological condition,BrEpDRF is signalled by NO-cGMP and PI3/Akt pathway. |
| Keywords: | bronchial epithelium derived relaxing factor （ BrEpDRF ） nitric oxide （NO） nitric oxide synthase （NOS） phosphatidylinositol 3-kinase/protein kinase B（PI3/Akt ） pathway cyclic guanosine 3＇ ,5＇-monophosphate （cGMP） |
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