| 结核分枝杆菌流行病学研究中基因分型的方法 |
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| 引用本文： | 韩晖,叶临湘. 结核分枝杆菌流行病学研究中基因分型的方法[J]. 国外医学:社会医学分册, 2006, 0(4) |
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| 作者姓名： | 韩晖 叶临湘 |
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| 作者单位： | 华中科技大学同济医学院公共卫生学院流行病与卫生统计学系 湖北武汉430030 |
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| 摘 要： | 限制性片段长度多态性(IS6110-RFLP)是结核分枝杆菌(MTB)基因分型的金标准,该方法分辨力高,但操作非常复杂和结果分析困难,因而出现了多种PCR基础的替代方法,如混合接头PCR、间隔区寡聚核苷酸分型(spoligotyping)、随机扩增多态性DNA(RAPD)和数目可变的串联重复序列和结核分枝杆菌散在分布重复单位(VNTR-MIRU)等。但除近来出现的MIRU-VNTR方法外,分辨力均不够强,上述两种或多种PCR方法联合可获得高的分辨力。
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| 关 键 词： | 结核 流行病 基因分型 |
The Gene-typing Methods of Mycobacterium Tuberculosis in Molecular Epidemiology |
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| HAN Hui,YE Linxiang. The Gene-typing Methods of Mycobacterium Tuberculosis in Molecular Epidemiology[J]. Foreign Medical Sciences(Section of Social Medicine), 2006, 0(4) |
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| Authors: | HAN Hui YE Linxiang |
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| Affiliation: | HAN Hui,YE Linxiang.Department of Epidemiology and Health Statistics,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430030,China |
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| Abstract: | IS6110-RFLP is the gold standard for the gene-typing of MTB.It has high-differentiation,but it's very complex for operation and difficult for data analysis,so there are some easier methods based on PCR including Mixed-linker PCR,such as Spoligotyping,RAPD,VNTR-MIRU and etc.Except the method of VNTR-MIRU,the ability of differentiation for other methods are not high,however,the combined method of two or more PCR methods can increase the differentiation. |
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| Keywords: | Tuberculosis Epidemiology Gene-typing |
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