大肠癌组织中胃泌素与癌基因c-myc、c-fos、ras p21表达和AgNORs计数的关系

目的 探讨大肠癌组织中胃泌素与癌基因ｃ－ｍｙｃ、ｃ－ｆｏｓ、ｒａｓ ｐ２１表达和核仁组成区嗜银染色（ＡｇＮＯＲｓ）计数的关系及其意义。方法 对４８例大肠癌病人癌组织进行检测，采用免疫组化Ｓ－Ｐ法测胃泌素、ｃ－ｍｙｃ、ｃ－ｆｏｓ、和ｒａｓ ｐ２１表达，改良银染法测ＡｇＮＯＲｓ计数，并与癌旁３ｃｍ、６ｃｍ粘膜及正常大肠粘膜作对照。结果 癌组织胃泌素、ｃ－ｍｙｃ、ｃ－ｆｏｓ、ｒａｓ Ｐ２１表达的阳性率和

Relationship of the expression of gastrin and oncogene c-myc,c-fos,ras p21 with AgNORs counting in colorectal carcinoma tissue

Objective To study the relationship of the expression of gastrin and oncogene c myc , c fos , ras p21 with AgNORs counting in colorectal carcinoma tissue and its clinical significance. Methods The expression of gastrin and c myc, c fos ras p21 of carcinoma tissue in 48 cases were detected with S P immunocytochemical method. The argyrophilia nucleolar organizer regions (AgNORs) was counted with modified Ag stain and, their expression in the mucosa 3 cm, 6 cm adjacent to carcinoma tissue and normal colorectal mucosa were also counted to be used in comparison. Results The positive expression rate of gastrin, c myc, c fos and ras p21 and AgNORs counting in carcinoma tissue were significantly higher than that in mucosa 3 cm, 6 cm adjacent to carcinoma tissue and normal colorectal mucosa( P <0.01 all). The positive expression rate of gastrin was significantly higer in highly differentiated adenocarcinoma group than in poorly differentiated and mucinous adenocarcinoma group( P <0.05). The positive expression rate of c myc and c fos and AgNORs counting were siginificantly higher in gastrin positive group than in gastrin negative group( P <0.05, P <0.01). Conclusion Certain colorectal carcinoma cells can form and secrete gastrin through autocrine and mediate the proliferation of carcinoma cells through the modulation of the amplification of oncogene c myc, c fos and ras p21.