耐甲氧西林金黄色葡萄球菌耐药性，SCCmec分型及毒素基因的检测

目的调查北京大学第一医院致病性耐甲氧西林金黄色葡萄球菌SCCmec分型情况、耐药状况、PVL、TSST-1基因携带率，初步了解临床耐甲氧西林金黄色葡萄球菌的耐药原因，为临床感染控制提供依据。方法收集2007年9月至2008年3月住院患者首次分离的53株有致病意义MRSA，用全自动微生物分析仪进行药敏试验，应用PCR方法检测MBSA的SCCmec基因型、PVL、TSST-1基因。结果53株MRSA呈多重耐药特点，SCCmec分型以ⅢA为主（47．2％），PVL基因均为阴性，12株（22％）MRSA TSST-1阳性。结论53株MILSA的多重耐药性与SCCmec结构密切相关，SCCmec分型技术是探索MRSA多重耐药性与耐药结构有效的分子生物学手段，TSST-1基因阳性株在临床分离的金黄色葡萄球菌中占有较高的比例。

Detection Antibiotic Resistance,SCCmec typing and toxic genes of Methicillin-Resistant Staphylococcus aureus

Objective To investigate the genotype of Staphylococcal chromosomal cassette mec （SCCmec）, and antibiotic resistance,panton-valentine leukocidin gene （PVL） and Toxic Shock Syndrome Toxin 1 Gene （TSSF-1） of clinical methicillin-resistant Staphylococcus aureus （MRSA） isolates in Peking University First Hospital, preliminarily find out multi-drug resistance reason and provide molecular epidemiological evidence for strategies to control MRSA infection .Methods 53 isolates of nosogenetic MRSA were collected from Sep 2007 to Mar 2008. The antimicrobial susceptibility test was proceeded by automated Microbiology Analyzer, SCCmec typing was determined by multiplex PCR,PVL and TSST-1 were confirmed by PCR.Results 53 isolates of MRSA are multiresistant, SCCmec typing show that most isolates am m A type（47.2% ） ,PVL gene in 53 MRSA isolates am all negative,the rate of TSST-1 ＋ is 22%. Conclusion The multiresistant characteristic of 53 MRSA isolates closely connect with SCCmec typing,SCCmec typing is a research mean for exploring the relationship between the structure and the mechanism of multi-drug resistance, TSST-1 gene positive strains were in a high proportion in the clinical isolates of S. aureus.