| 高效液相色谱法测定更年宁心片中盐酸小檗碱的含量 |
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| 引用本文： | 谭颖丹. 高效液相色谱法测定更年宁心片中盐酸小檗碱的含量[J]. 实用药物与临床, 2008, 11(5) |
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| 作者姓名： | 谭颖丹 |
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| 作者单位： | 盘锦市第四人民医院,辽宁,盘锦,124000 |
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| 摘 要： | 目的建立测定更年宁心片中盐酸小檗碱含量的高效液相色谱法。方法以十八烷基硅烷键合硅胶为填充剂;以乙腈-水(每100mL加0.26mL磷酸、0.5mL三乙胺与0.05g十二烷基硫酸钠)(46:54)为流动相;检测波长为264nm。结果盐酸小檗碱在8.02～72.18μg/mL范围内线性关系良好,平均回收率为100.19%,RSD为1.21%(n=5)。结论该方法稳定,重现性好,可作为更年宁心片中盐酸小檗碱的含量测定方法。
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| 关 键 词： | 高效液相色谱法 更年宁心片 盐酸小檗碱 |
Determination of Berberine Hydrochloride in Gengnian ningxin tablets by HPLC |
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| TAN Ying-dan. Determination of Berberine Hydrochloride in Gengnian ningxin tablets by HPLC[J]. Practical Pharmacy and Clinical Remedies, 2008, 11(5) |
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| Authors: | TAN Ying-dan |
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| Abstract: | Objective To establish an HPLC method for determination of Berberine Hydrochloride in Gengnian ningxin tablets.Methods Symetry C18 column was used,and the mobile phase was the mixture of acetonitrile and water(0.26 mL phosphoric acid,0.5 mL triethylamine and 0.05 g sodium dodecyl sulfate in 100 mL water,46:54),detection wavelength was at 264 nm.Results The linear range was 8.02～72.18 μg/mL,and the average recovery was 100.19%,RSD=1.21%(n=5).Conclusion The method is accurate,reproducible,and it can be used to determine Berberine Hydrochloride in Gengnian ningxin tablets. |
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| Keywords: | HPLC Gengnian ningxin tablets Berberine Hydrochloride |
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