| 恶性疟原虫海南、云南、安徽株裂殖子表面抗原MSA1第二区基因的克隆和序列分析 |
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| 引用本文： | 李明,谢毅,李英杰,任大明. 恶性疟原虫海南、云南、安徽株裂殖子表面抗原MSA1第二区基因的克隆和序列分析[J]. 中国医学科学院学报, 1996, 0(3) |
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| 作者姓名： | 李明 谢毅 李英杰 任大明 |
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| 作者单位： | 第一军医大学热带医学研究所，复旦大学遗传工程国家重点实验室 |
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| 摘 要： | 应用ＰＣＲ技术扩增并克隆恶性疟原虫海南、云南、安徽３个地理株ＭＳＡ１第二区基因，测定并分析其基因序列。结果表明，海南、云南、安徽株扩增片段长度为４２３ｂｐ，基因序列完全相同，我国虫株ＭＳＡ１与ＷＥＬＬＣＯＭＥ株最为相似，存在ＭＡＤ和Ｋ１两种等位基因型的基因内重组现象。
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| 关 键 词： | 恶性疟原虫，裂殖子表面抗原，基因序列分析 |
Cloning and Sequence Analysis of the Gene Encoding the Second Region of MSA1 from Three Chinese Isolates of Plasmodium falciparum |
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| Li Ming,Xie Yi,Li Yingjie,Ren Daming. Cloning and Sequence Analysis of the Gene Encoding the Second Region of MSA1 from Three Chinese Isolates of Plasmodium falciparum[J]. Acta Academiae Medicinae Sinicae, 1996, 0(3) |
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| Authors: | Li Ming Xie Yi Li Yingjie Ren Daming |
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| Abstract: | Gene encoding the second region of merozoite surface antigen(MSA1-R2)of three Chinese isolates of Plasmodium falciparum from Hainan,Yunnan and Anhui were amplified by PCR,cloned into M13 and sequenced.Comparison among MSA1-R2 sequences from different isolates of P.falciparum shows that there is no difference among the three Chinese isolates.The sequence of MSA1-R2 of the Chinese isolates are highly homologous with that of WELLCOME from West Africa.The sequence flanking the upstream of the tandem repeats is exactly the same as that of MAD-20,but sequence downstream the repeats exhibit the characteristics of K1 allele,indicating that intragenic recombination of the two MSA1 allelic families exists in the Chinese isolates. |
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| Keywords: | Plasmodium falciparum merozoite surface antigen-1 sequence analysis |
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