| 免疫印迹法与间接免疫荧光法检测抗核抗体对照分析 |
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| 引用本文： | 张铁汉,赵永新,郑辉,王伟娟.免疫印迹法与间接免疫荧光法检测抗核抗体对照分析[J].中国热带医学,2008,8(4):568-570. |
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| 作者姓名： | 张铁汉 赵永新 郑辉 王伟娟 |
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| 作者单位： | 新乡医学院第三附属医院检验科,河南,新乡,453003 |
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| 摘 要： | 目的探讨欧蒙免疫印迹法不同项目阳性与间接免疫荧光法检测抗核抗体（ANA）不同核型的相关性。方法对比分析251例欧蒙印迹法不同项目阳性病例的抗体谱类型与其ANA阳性核型。结果欧蒙印迹法抗体类型不同，ANA荧光核型有很大差别，规律不明显。结论依据荧光核型来推断抗ENA等抗体不可靠。
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| 关 键 词： | 抗核抗体 抗可提取性核抗原 自身抗体 免疫印迹 |
| 文章编号： | 1009-9727(2008)4-568-03 |
| 修稿时间： | 2008年1月21日 |
Comparison of detection of serum antinuclear antibody with immunoblotting and indirect immunofluorescence (IIF) |
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| Institution: | ZHANG Tie - han, ZHAO Yong - xin, ZHENG Hui, et.al(Third Affiliated Hospital of Xinxiang Medical College, Xinxiang453003, Henan, P. R. China) |
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| Abstract: | Objective To investigate the correlation of positive types of ANA Profile 3 EUROLIN and their patterns of the antinuclear antibodies（ANA） by IIF in human serum. Methods Indirect immunofluorescence （IIF） （by HEP -2 cells and primate liver as antigen substrates） and ANA Profile 3 EUROLIN antinuclear antibodies were used to analyze 251 patterns of the ANA and their antibody types respectively. Results There no obvious relationship between positive types of ANA Profile 3 EUROLIN antibodies and their patterns of the ANA by IIF in human serum was observed. Coneluslon The patterns of ANA by IIF cannot be used to conclude the types of ANA Profile 3 EUROLIN. |
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| Keywords: | Antinuclear Antibodies Extractable nuclear antigen Autoantibodies immuoblotting |
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