| 沙眼衣原体L2型MOMP基因真核表达载体的构建及序列分析 |
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| 引用本文： | 彭辉 宁波波 等. 沙眼衣原体L2型MOMP基因真核表达载体的构建及序列分析[J]. 中国人兽共患病杂志, 2001, 17(3): 52-54 |
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| 作者姓名： | 彭辉 宁波波 等 |
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| 作者单位： | 彭辉（中山医科大学免疫学教研室广州，510089） 宁波（中山医科大学免疫学教研室广州，510089） 冯炼强（中山医科大学免疫学教研室广州，510089） 张春艳（中山医科大学免疫学教研室广州，510089） 徐霖（中山医科大学免疫学教研室广州，510089） |
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| 摘 要： | 目的 构建携带沙眼衣原体L2型主要外膜蛋白（MOMP）基因的真核表达载体，为沙眼衣原体的DNA疫苗的研究提供材料。方法 用PCR技术扩增L2型沙眼衣原体MOMP基因片段，再将其定向插入到真核表达载体pcDNA3多克隆位点中，构建成重组表达质粒。并用酶切分析、PCR扩增及部分序列测定等方面对重组质粒进行了鉴定。结果 MOMP基因被正确地克隆到直核表达载体pcDNA3中。测序结果同文献报道的序列一致。结论 真核细胞表达载体pcDNA3－MOMP的成功构建，为进一步开展沙眼衣原体的DNA疫苗的研究奠定了基础。
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| 关 键 词： | 沙眼衣原体 主要外膜蛋白 克隆 DNA疫苗 真核表达载体 序列分析 |
| 文章编号： | 1002-2694(2001)03-0052-03 |
| 修稿时间： | 2000-07-08 |
CONSTRUCTION OF AN EUKARYOTIC EXPRESSION VECTOR PCDNA3-MOMP CARRYING THE MAJOR OUTER MEMBRANE PROTEIN GENE FROM CHLAMYDIA TRACHOMATIS SEROVAR L2 AND PARTIAL SEQUENCE DETERMINATION |
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| PENG Hui,NING Bo,FENG Lianqiang,ZHANG Chunyan,XU Lin. CONSTRUCTION OF AN EUKARYOTIC EXPRESSION VECTOR PCDNA3-MOMP CARRYING THE MAJOR OUTER MEMBRANE PROTEIN GENE FROM CHLAMYDIA TRACHOMATIS SEROVAR L2 AND PARTIAL SEQUENCE DETERMINATION[J]. Chinese Journal of Zoonoses, 2001, 17(3): 52-54 |
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| Authors: | PENG Hui NING Bo FENG Lianqiang ZHANG Chunyan XU Lin |
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| Abstract: | Aim To construct an eukaryotic expression vector carrying the Chlamydia trachomatis major outer membrance protein (MOMP) gene.Methods The MOMP gene was amplified from the genome of the Chlamydia trachomatis serovar L2 by PCR and recombined with plasmid pcDNA3.The recombinant plasmid pcDNA3-MOMP was verified with restriction analysis,PCR and partial sequencing.Results MOMP gene was cloned correctly into vector pcDNA3.The resultant sequence is completely consistent with the published sequence.Conclusion The eukaryotic expression vector pcDNA3-MOMP was constructed successfully for research on DNA vaccine of Chlamydia trachomatis. |
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| Keywords: | Chlamydia trachomatis Major outer membrane protein Cloning DNA vaccine |
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