高效液相色谱法测定氨酪酸氯化钠注射液中的α-吡咯烷酮 |
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引用本文: | 许惠方,李玉梅. 高效液相色谱法测定氨酪酸氯化钠注射液中的α-吡咯烷酮[J]. 中国医药科学, 2014, 0(22): 87-89 |
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作者姓名: | 许惠方 李玉梅 |
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作者单位: | 广东省博罗县人民医院 |
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摘 要: | 目的建立高效液相色谱法检测氨酪酸氯化钠注射液中α-吡咯烷酮含量的方法标准。方法选用CAPCELL PAK C18柱为色谱柱;流动相采用p H2.1的磷酸二氢钾溶液-甲醇(90:10);流速维持在1.0m L/min;紫外检测波长设定为210nm;柱温为30℃;进样量为10μL。结果α-吡咯烷酮浓度为0.4-5.0mg/L时其线性关系良好,r=0.9998,平均回收率为100.27%(RSD=0.54%,n=6)。结论本研究建立的HPLC法对于氨酪酸氯化钠注射液中的α-吡咯烷酮测定效果良好且方便快捷。
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关 键 词: | 氨酪酸氯化钠注射液 α-吡咯烷酮 含量测定 高效液相色谱法 |
Determination of aminobutyric acid in aminobutyric acid sodium chloride injection by HPLC |
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Affiliation: | XU Huifang ,LI Ymnei( People's Hospital of Boluo County in Guangdong Province, Boluo 516100, China) |
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Abstract: | Objective To establish an HPLC standard method to assay the α-pyrrolidone content in aminobutyric acid sodium chloride injection. Methods A CAPCELL PAK C18 column was adopted,and potassium dihydrogen phosphate solution were diluted with water to 1000 m L as mobile phase,which adjusted to p H 2.1 with phosphoric acid)-methanol(90:10,v/v).The flow rate was 1.0m L/min,the detection wavelength was 210 nm and column temperature was 30℃.Results The linear correlation of aminobutyric acid was fine within 0.4-5.0mg/L(r=0.9998). The average recovery was 100.27%(RSD=0.54%,n=6). Conclusion The HPLC method we established was good and convenient to determine the aminobutyric acid contents in amino butyric acid sodium chloride injection. |
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Keywords: | Aminobutyric acid sodium chloride injection α-pyrrolidone Content determination HPLC |
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