银染端粒重复序列扩增法检测肺癌端粒酶活性

目的 评价端粒酶能否作为肺癌诊断的一具肿瘤标志物。方法 采用改良的银染端粒重复序列扩增法（ＴＲＡＰ）检测３４例人肺癌手术标本（包括癌组织、相应癌旁组织与正常组织标本各３４份，２８个相应淋巴结）的端粒酶活性。另有肺囊肿、肺炎性假瘤各３例作为对照。结果 ８８．３％（３０／３４）的肺癌癌组织端粒酶阳性，正常组织全部阴性。结论 端粒酶可以作为肺癌诊断的一人肿瘤标志物，改良的银染ＴＲＡＰ法成本低，污染少，有

Detection of telomerase activity in human lung cancer by silver staining telomeric repeat amplification protocol

Objective To study the telomerase activity in lung cancer and identify whether it can be a specific tumor marker or not. Methods Telomerase activity was measured in the specimens of lung cancer tissue, adjacent tissue, normal tissue and lymph nodes from 34 primary lung cancer patients by PCR based silver staining telomeric repeat amplification protocol(TRAP). Control study was made on the specimens taken from 6 patients with benign lung lesions (3 lung cysts and 3 tumorous inflammatory lesions).Results The telomerase activity was positive in 88.3%(30/34) lung cancer specimens,5.8%(2/34) their adjacent tissue specimens and 18.5%(8/28) lymph nodes specimens . No telomerase activity was found in normal tissues. Only 16.7%(1/6) benign lung lesions showed telomerase activity. Conclusion Telomerase activity may be a specific tumor marker in diagnosis of lung cancer. The non-radioisotopic silver staining TRAP, cheap in cost and causing no radio active pollution,is of practical value clinically.