| 肝纤维化大鼠肝窦内皮细胞整合素α6β1及粘着斑激酶表达的变化 |
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| 作者单位： | 刘小菁（610041,成都,华西医科大学附属第一医院内科实验室）;傅华（610041,成都,华西医科大学附属第一医院心内科）;杨丽（610041,成都,华西医科大学附属第一医院消化内科）;黄明慧（610041,成都,华西医科大学附属第一医院内科实验室）;肖文君（610041,成都,华西医科大学附属第一医院消化内科）;王一平（610041,成都,华西医科大学附属第一医院消化内科） |
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| 基金项目： | 国家自然科学基金(39700068) |
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| 摘 要： | 目的研究正常及肝纤维化时大鼠肝窦内皮细胞(SEC)表面层粘连蛋白(LN)的整合素受体α6 β 1及粘着斑激酶(FAK)的变化.方法用胶原酶原位灌注、Percoll不连续密度梯度离心法分离正常及四氯化碳(CCl4)实验性大鼠肝纤维化模型中的SEC,并进行体外培养.采用细胞-ELISA和免疫沉淀-蛋白质酪氨酸激酶活性测定的方法,分别观察SEC细胞表面整合素α6 β 1表达及FAK活性的变化.结果正常大鼠SEC细胞表面几乎不表达整合素α6β 1;在肝纤维化时SEC细胞表面α 6 β 1蛋白表达却明显增加(P＜0.05),且细胞中FAK活性明显增高(P＜005).结论在肝纤维化时SEC细胞表面整合素α 6 β 1的表达及FAK活性明显增高,可能在SEC的形态及功能改变中起重要作用.
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| 关 键 词： | 肝纤维化 肝窦内皮细胞 整合素 粘着斑激酶 大鼠 |
| 修稿时间： | 2000-11-17 |
Changes of the expression of integrin |
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| X Liu,H Fu,L Yang,M Huang,W Xiao,Y Wang. Changes of the expression of integrin[J]. Chinese journal of hepatology, 2001, 9(6): 349-351 |
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| Authors: | X Liu H Fu L Yang M Huang W Xiao Y Wang |
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| Affiliation: | Department of Internal Medicine, First Affiliated Hospital, West China University of Medical Sciences, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To investigate the expression of integrin alpha(6)beta(1) and the activity of focal adhesion kinase (FAK) in liver sinusoidal endothelial cells (SECs) from experimental fibrotic rats induced by CCl(4). METHODS: By in situ collagenase perfusion and two-step Percoll gradient centrifugation, SECs were isolated and cultured from normal and CCl(4) -treated Wistar rats. The expression of integrin alpha(6)beta(1) was determined by cell-ELISA, and the activity of FAK was assessed by immunoprecipitation-tyrosine kinase assay. RESULTS: The integrin alpha(6)beta(1) was almost absent in the normal SECs and was up-regulated during the fibrotic process; SECs from experimental fibrotic rats possessed higher expression level of integrin alpha(6)beta(1) than normal SECs (P<0.05). The FAK activity in SECs from experimental fibrotic rats increased significantly as compared with the normal controls (P<0.05). CONCLUSIONS: The expression of integrin alpha(6)beta(1) on SECs and the increase of FAK in SECs may be important in the phenotype and function changes of SECs during hepatic fibrogenesis. |
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