| 大肠杆菌poly(A)化mRNA cDNA文库的构建与鉴定 |
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| 引用本文： | 胡子有,马文丽,吴清华,张宝,宋艳斌,郭秋野,郑文岭.大肠杆菌poly(A)化mRNA cDNA文库的构建与鉴定[J].南方医科大学学报,2002,22(11):1005-1007. |
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| 作者姓名： | 胡子有 马文丽 吴清华 张宝 宋艳斌 郭秋野 郑文岭 |
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| 作者单位： | 1. 第一军医大学分子生物学研究所,广东广州,510515
2. 广州军区总医院分子肿瘤研究所,广东,广州,510010 |
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| 基金项目： | 广州市重点科技攻关项目(99-Z-022-01) |
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| 摘 要： | 目的构建和鉴定大肠杆菌poly(A)化mRNA的cDNA文库。方法应用限制性显示PCR技术构建大肠杆菌poly(A)化mRNA的cDNA文库,筛选、收集cDNA片段,并进行测序。根据测序结果进行初步的生物信息学分析和结果验证。结果构建了大肠杆菌poly(A)化mRNA的限制性cDNA文库,并对其中66个基因片段进行了分析。结论所构建的大肠杆菌poly(A)化mRNA的cDNA文库片段重复性低,质量高。
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| 关 键 词： | DNA 互补 大肠杆菌 克隆 分子 多聚腺苷酸化 |
| 文章编号： | 1000-2588(2002)11-1005-03 |
| 修稿时间： | 2002年3月28日 |
Construction and identification of cDNA library ofE. coli mRNA with poly(A) tracts |
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| HU Zi-you ,MA Wen-li ,WU Qing-hua ,ZHANG Bao ,SONG Yan-bin ,GUO Qiu-ye ,ZHENG Wen-ling Institute of Molecular Biology,First Military Medical University,Guangzhou ,China.Construction and identification of cDNA library ofE. coli mRNA with poly(A) tracts[J].Journal of Southern Medical University,2002,22(11):1005-1007. |
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| Authors: | HU Zi-you MA Wen-li WU Qing-hua ZHANG Bao SONG Yan-bin GUO Qiu-ye ZHENG Wen-ling Institute of Molecular Biology First Military Medical University Guangzhou China |
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| Institution: | HU Zi-you 1,MA Wen-li 1,WU Qing-hua 1,ZHANG Bao 1,SONG Yan-bin 1,GUO Qiu-ye 1,ZHENG Wen-ling 21 Institute of Molecular Biology,First Military Medical University,Guangzhou 510515,China, 2 Institute of Molecular Oncology,Guangzhou General Hospital of Guangzhou Co mmand,Guangzhou 510010,China |
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| Abstract: | Objective To construct and identify th e cDNA library of E.coli mRNA with poly(A) tracts. Methods The cDNA library of E .coli was constructed by restriction display-PCR (RD-PCR) technique, followed by sequencing and bioinformatics analyses. Results cDNA library of E.coli mRNA w ith poly(A) tracts was successfully constructed, and 66 gene fragments were seq uenced. Conclusion The constructed cDNA library of E.coli mRNA with poly(A) tra cts contains alow rate of repeti-tion and is of high quality. |
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| Keywords: | DNA comp lementary Escherichia coli cloning molecular polyadenylation |
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