上皮性卵巢癌组织中乙酰肝素酶的表达及其与预后的关系

目的 研究乙酰肝素酶(Hpa)在上皮性卵巢癌组织中的表达,分析其与卵巢癌临床病理特征及预后的关系.方法 采用RT-PCR和免疫组化方法检测乙酰肝素酶mRNA及蛋白在上皮性卵巢癌、卵巢良性肿瘤和正常卵巢组织中的表达.结果 Hpa mRNA在卵巢癌组织中的阳性表达率为73.33%(33/45),相对表达水平为0.65±0.37,均明显高于卵巢良性肿瘤和正常卵巢组织(0.39±0.49/0.31±0.52),差异有显著性(P均<0.05).Hpa蛋白主要定位于细胞胞质,在卵巢癌组织中染色以(++)-(+++)为主(44.44%),明显强于卵巢良性肿瘤(13.33%)和正常卵巢组织(0.00%).有淋巴结转移的癌组织Hpa蛋白及mRNA水平均高于无淋巴结转移组,FIGO分期Ⅲ-IV的癌组织表达高于I-Ⅱ组,均有统计学意义(P<0.01).Log-rank 分析显示Hpa 表达阳性者的累积生存率明显低于阴性者,差异有显著性(P<0.05).结论 Hpa在上皮性卵巢癌组织中高表达,并且与卵巢癌的临床分期、淋巴结转移及病人的生存期密切相关,可作为判断上皮性卵巢癌生物学行为和预后的重要参考指标.

The expression of heparanase in epithelial ovarian cancer and its correlation with the prognosis

Objective:To investigate the expression of heparanase(Hpa)in epithelial ovarian cancer and its correlation with clinical pathological feature and prognosis of the patients.Methods: Expression of Hpa in ovarian cancers,benign ovarian tumor and normal ovarian tissues were detected by RT-PCR assay and immunohistochemical staining.Results: Positive expression rate of Hpa in tumor tissues was 73.33%,significantly higher than that in benign tumors and normal tissues(P<0.05).Hpa protein-positive mainly located in cell kytoplasm,staining intensity mainly was(++)-(+++),significantly stronger than that in control group.The expression of Hpa and was higher in cancerous tissues with lymphatic metastasis than that without lymphatic metastasis(P<0.01).The positive rate of Hpa in stage III-IV was higher than that of patients in stage I-II(P<0.01).Log-rank analyzis indicated the accumulated survival rate of the patients with Hpa expression was lower than the patients without Hpa expression(P<0.05).Conclusion: The overexpression of Hpa may play an important role in the development and progression of ovarian tumors.The expression of Hpa can be used as an useful marker of the biological behavior of ovarian cancer and an indepent prognostic factor for ovarian cancer.