CXCR4单克隆抗体抑制乳腺癌MCF-7细胞裸鼠移植瘤的实验研究

目的 研究CXC趋化因子受体4(CXC chemokine receptor 4,CXCR4)单克隆抗体(CXCR4 mAb)对人乳腺癌MCF-7细胞裸鼠皮下移植瘤生长的影响,并初步探讨CXCR4 mAb抗肿瘤的作用机制.方法 采用8周龄Balb/c雌性裸鼠,建立乳腺癌MCF-7细胞裸鼠皮下移植瘤模型.运用CXCR4 mAb进行干预,从整体水平观察CXCR4 mAb对肿瘤生长的影响,采用免疫组织化学法检测肿瘤组织中增殖细胞核抗原(PCNA)、半胱氨酸天冬氨酸酶3(Caspase-3)和血管内皮细胞生长因子(VEGF)的表达情况.结果 CXCR4 mAb可明显抑制移植瘤的生长,瘤体抑制率达到71.4%;CXCR4 mAb治疗后的肿瘤组织中PCNA和VEGF表达明显下降,而Caspase-3表达上升.结论 CXCR4 mAb可能是通过抑制肿瘤细胞增殖、促进肿瘤细胞凋亡及抑制肿瘤血管形成而发挥抗肿瘤生长的作用.

CXCR4 monoclonal antibody inhibits the growth of MCF-7 breast cancer xenograft in nude mice

Objective:To investigate the effect of CXCR4 monoclonal antibody(CXCR4 mAb)on the growth of human MCF-7 breast cancer xenograft subcutaneously implanted in nude mice and to explore the antitumor mechanism of CXCR4 mAb.Methods: 8-week-old Balb/c femal nude mice were adopted to establish a subcutaneously tumor-bearing model.Treating animals with CXCR4 mAb.The inhibitory effects of CXCR4 mAb on tumor growth were assessed after treatment.Immunohistochemical staining was performed to examine the expressions of PCNA,Caspase-3 and VEGF.Results: CXCR4 mAb significantly inhibited the growth of breast cancer xenograft in nude mice.Inhibition ratio of tumor volum reached 71.4%.After treatment,CXCR4 mAb markedly downregulated the expressions of PCNA and VEGF while upregulated the expression of Caspase-3.Conclusion: CXCR4 mAb might inhibit breast cancer growth in vivo via the induction of cancer cell apoptosis and inhibition of cancer cell proliferation and tumor angiogenesis.