miR-27a下调肾癌细胞FOXO1表达并促进其细胞增殖

目的 探讨microRNA-27a(miR-27a)在肾癌细胞中的作用及调控机制.方法 应用miR-27a反义寡核苷酸(ASO)在体外转染786-O和Caki-1细胞;qRT-PCR法检测miR-27a及FOXO1 mRNA的表达情况,Western blot检测FOXO1蛋白的表达水平;CCK8检测miR-27a ASO对细胞增殖的影响.结果 786-O和Caki-1细胞中miR-27a表达高于HK2正常肾小管上皮细胞(P<0.05);利用miR-27a ASO抑制786-O和Caki-1细胞miR-27a表达后,发现两个细胞株FOXO1 mRNA和蛋白表达的升高及增殖能力的降低(P<0.05).结论 miR-27a 可能通过调控FOXO1表达在肾癌中起致癌作用.miR-27a ASO可抑制肾癌细胞的增殖,因此miR-27a有可能作为肾癌基因治疗的候选靶点.

miR-27a promotes the renal cell carcinoma cell proliferation though down-regulating FOXO1

Objective:To study the function and regulation mechanisms of miR-27a in renal cell carcinoma cell lines.Methods:Cells of 786-O and Caki-1 were transfected with miR-27a antisense oligonucleotides(ASO)in vitro.Quantitative real-time PCR was used to examine the expression levels of miR-27a and FOXO1 mRNA;Western blot was used for detecting the FOXO1 protein expression;CCK8 assay was used to determine the influence of miR-27a ASO on cell growth.Results:The expression levels of miR-27a in cells of 786-O and Caki-1 were higher than that in HK2 normal renal epithelial cell(P<0.05).After decreasing miR-27a using miR-27a ASO in cells of 786-O and Caki-1,the levels of FOXO1 mRNA and protein were increased,and the proliferation ability was inhibited(P<0.05).Conclusion:miR-27a may play an important role in renal carcinogenesis though regulating FOXO1 expression.miR-27a ASO could suppress the growth of renal cell carcinoma cells,so miR-27a may be a candidate target for gene therapy of renal cell carcinoma.