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| 子宫内膜癌中microRNA-125b对HER2基因3' 非翻译区的调控作用 | |
| 引用本文： | 孟丽荣,鲁艳明,尚超.子宫内膜癌中microRNA-125b对HER2基因3' 非翻译区的调控作用[J].现代肿瘤医学,2012,20(1):18-20. |
| 作者姓名： | 孟丽荣 鲁艳明 尚超 |
| 作者单位： | 1. 澳门理工学院,高等卫生学校,澳门,000853 2. 中国医科大学附属盛京医院妇产科,辽宁,沈阳,110004 3. 中国医科大学神经生物学教研室,辽宁,沈阳,110001 |
| 基金项目： | 澳門理工學院科研基金資助项目(编号:RP/ESS-03/2009) |
| 摘 要： | 目的:明确miR-125b在子宫内膜癌中对HER2基因3'UTR的靶向调控作用.方法:分别构建野生型和突变型HER2基因 3'UTR荧光素酶载体;软件预测HER2基因 3'UTR靶向miRNAs;共转染miR-125b和荧光素酶载体于HEC-1B细胞中,双荧光素酶检测系统测定荧光素酶活性.结果:通过软件预测在HER2 3'UTR第37bp位置有一个miR-125b结合位点;与转染对照miR组和突变型载体组相比,miR-125b前体能明显降低野生型载体的荧光素酶活性.结论:miR-125b能够靶向负性调控HER2基因 3'UTR活性. |
| 关 键 词： | 子宫内膜癌 miR-125b HER2 3'UTR |
The role of microRNA-125b on HER2 gene 3'-untranslated region in endometrial cancer |
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| MENG Lirong,LU Yanming,SHANG Chao.The role of microRNA-125b on HER2 gene 3'-untranslated region in endometrial cancer[J].Journal of Modern Oncology,2012,20(1):18-20. | |
| Authors: | MENG Lirong LU Yanming SHANG Chao |
| Institution: | 1School of Health Sciences,Macao Polytechnic Institute,Macao 000853,China;2Department of Gynaecology and Obstetrics,The Affiliated Shengjing Hospital,China Medical University,Liaoning Shenyang 110004,China;3Department of Neurobiology,China Medical University,Liaoning Shenyang 110001,China. |
| Abstract: | Objective:To identify the regulation of miR-125b to HER2 gene 3’UTR in endometrial cancer.Methods: The wild type and mutation type of HER2 gene 3’UTR luciferase vectors were constructed.Software was applied to predict the HER2 3’UTR targeting miRNAs.The dual luciferase assay system was used to detect the reporter activity followed by co-transfecting the luciferase vector constructed and pre-miRNA into HEC-1B cells.Results: MiRNA targeting prediction showed that the 37th bp in 3’UTR contained a miR-125b binding site.The luciferase assay revealed that pre-miR-125b could significantly decrease the luciferase activity of wild type HER2 3’UTR vector,compared with the control and mutation vector transfection groups,P<0.05.Conclusion: MiR-125b could negatively target and regulate the activity of HER2 gene 3’UTR. |
| Keywords: | endometrial cancer miR-125b HER2 3’UTR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |