HIV infection and aging: enhanced Interferon- and Tumor Necrosis Factor-alpha production by the CD8+ CD28- T subset |
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| Authors: | Edward H Eylar Carmen E Lefranc Yasuhiro Yamamura Ineabely Báez Sol Luis Colón-Martinez Nayra Rodriguez T B Breithaupt |
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| Institution: | (1) Biochemistry Department, Ponce School of Medicine, P.O. Box 7004, Ponce, PR 00732-7004, USA;(2) Des Moines University-Osteopathic Medical Center, Des Moines, IA 50312, USA |
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| Abstract: | Background T cells from HIV+ and aged individuals show parallels in terms of suppressed proliferative activity and interleukin-2 (I1-2) production and an increased number of CD8+ CD28- T cells. In order to compare cytokine production from T cells from these two states, CD4+ and CD8+ T cells from HIV+ aged, and normal young donors (controls) were monitored for cytokine production by flow cytometry, quantitative PCR and ELISA upon activation by PMA and anti-CD3. In addition, the CD8+ T cell subsets CD28+ and CD28- from the HIV+ and the aged groups were evaluated for cytokine production by flow cytometry, and compared with those from young controls. |
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