脉络宁注射液对面肌痉挛兔面神经内降钙素基因相关肽表达的影响

目的 探讨脉络宁注射液对面神经损伤的保护机制.方法 采用兔颞浅动脉压迫,已人为脱髓鞘变的周围面神经主干,通过电生理技术检测面肌痉挛特有的异常肌反应,建立兔面肌痉挛动物模型.用脉络宁注射液自耳缘静脉推注持续2周给药,采用免疫组化等方法,观察脉络宁注射液对面神经内降钙素基因相关肽（CGRP）表达的变化.结果 脉络宁组动物面神经内CGRP的表达明显高于模型组（P＜0.01）,假手术组动物面神经内未见CGRP免疫反应阳性纤维.模型组动物面神经纤维溃变明显,髓鞘松解分离,轴突肿胀有空泡或全部消失;脉络宁组动物面神经病变较轻.结论 脉络宁注射液对面肌痉挛脱髓鞘面神经有明显的保护作用,与其能促进面神经内CGRP的表达密切相关.

Effect of Mailuoning Injection on Calcitonin Gene-related Peptide Expression in Facial Nerve of Rabbits with Facial Spasm

ObjectiveTo investigate the mechanism of Mailuoning injection (MLN) in protecting facial nerve from injury. Methods The New Zealand white rabbit model with facial spasm was established by compressing superficial temporal artery to make artificial demyelinated lesion of the main peripheral facial nerve trunk. The successful establishment was confirmed by using electrophysiological technique to determine abnormal muscle response (AMR) which is a characteristic for facial spasm. MLN was injected continuously through ear marginal vein for 2 weeks.The change of CGRP expression in facial nerve was detected by immunohistochemical technique. Results As compared with the model group,CGRP expression in facial nerve was significantly increased in the MLN group (P<0.01),and CGRP immunoreactive positive fibers were not seen in the sham-operation group.In the model group, the facial nerve fibers degenerated obviously, myelin sheath loosened and dissociated, the turgent axons with vacuole or even completely disappeared. But the facial nerve lesion was lessened in the MLN group. Conclusion MLN has a significant protective effect on facial nerve demyelination in rabbits with facial spasm,which is closely related with its effect in improving CGRP expression in the facial nerve.