平肝潜阳药物对甲亢肝阳上亢证大鼠下丘脑蛋白质表达的影响

目的:观察平肝潜阳药物对甲亢肝阳上亢证大鼠下丘脑蛋白质表达的影响,为进一步探讨平肝潜阳药物治疗甲亢肝阳上亢证作用机制提供依据.方法:左旋甲状腺素(L-T4)腹腔注射和附子汤灌胃以建立甲亢肝阳上亢证模型,应用蛋白质组学技术比较正常组、甲亢肝阳上亢证组及平肝潜阳药物治疗组中下丘脑的差异蛋白质.结果:3组蛋白质斑点总体分布相似,主要分布在等电点pI 3～10,分子质量为13.8～98.8 kD.与正常组比较,甲亢肝阳上亢证模型组中蛋白质点表达上调的有6个点,下调的有10个点;在模型组中上调的6个点在治疗后均较治疗前有下降;而模型组中下调的10个点在治疗后均较治疗前有上调.结合生物信息学进行质谱鉴定,发现其中9个蛋白质分别为prohibitin,硫氧还蛋白过氧化物酶6,组氨酸三聚体核苷结合蛋白1,蛋白酪氨酸磷酸酶,假定蛋白,肌动蛋白抑制蛋白2,硫氧还蛋白过氧化物酶-Ⅱ,热休克蛋白-27,膜联蛋白-A1.结论:平肝潜阳药物治疗后的甲亢肝阳上亢证大鼠下丘脑蛋白质的表达具有差异,所鉴定的这9个蛋白质可能与平肝潜阳药物的作用机制有关.

Effect of pinggan qianyang on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang

OBJECTIVE: To investigate the effect of pinggan-qianyang (PGQY), a Chinese medicine, on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang, and to explore its mechanism. METHODS: The rat model was established by intraperitoneal injection of levo-thyroxine (L-T4) and fuzi decotion. All the quantitative and qualitative changes of the protein expressions were compared among the normal group,the model group and the treatment group by proteomic techniques. RESULTS: The protein spots in the 3 groups were mainly displayed at the isoelectric point (pI) 3 approximately 10, and the molecular weights were 13.8 approximately 98.8 kD.Compared with the normal group, 6 spots of protein expression increased and 10 decreased in the model group. All the changed protein in the model group returned to normal level after PGQY treatment. Mass-spectrometer and bio-informatics indicated that these proteins were Prohibitin, Peroxiredoxin-6, histidine triad nucleotide-binding protein 1, protein-tyrosine-phosphatase, predicted protein, profilin-2, peroxir doxin-II, heat shock protein-27, and annexin-A1. CONCLUSION: There are differences in the expression of hypothalamus proteins in the hyperthyroid rats with hyperactivity of liver-yang after the treatment with PGQY, and the 9 identified protein spots may be associated with the mechanism of PGQY.