光化学法致栓模型中血小板血栓生长速度的研究

本文应用光化学方法在大鼠肠系膜微血管内定位建立活体血小板血栓生成模型，通过生物荧光显微电视系统对整个实验过程进行观察和记录。利用视频多媒体系统和自行开发的计算机图象处理系统，对血栓生长的动态过程进行图象的采样、处理和分析，定量测量了血栓开始形成时间、血栓完全栓塞时间，以及归一化后的血栓长度、血栓高度、血管狭窄截面面积、血栓表面积和血栓体积等指标的时变曲线，并对光化学法致栓模型中血小板血栓的生长速度进行了讨论。本文提出的方法使定量描述血栓的动态生长过程成为可能，为客观评价各类病理条件或药物作用条件下的血栓生长情况提供了定量指标和比较依据

GROWTH RATE OF PLATELET THROMBUS IN A PHOTOCHEMICALLY INDUCED THROMBOSIS MODEL

Platelet thrombus in vivo was induced in rat mescnteric microvessel by irradiation with filtered light in combination with intravenous (i.v.) administration of fluorescent dye. The whole process of the experiment was visualized and recorded in the tape through an intravital fluorescent microscopic system. The dynamic process of thrombus formation was digitized using a multimedia computer system. With a specially developed image processing software, initiation time of thrombus formation, totally occluded time, and the time course changes of normalized thrombus length, thrombus height, cross area, thrombus surface area and thrombus volume were measured quantitatively. Besides, the growth rate of platelet thrombus in this thrombosis model was discussed. The method presented here will be useful for describing the dynamic process of thrombus formation, and evaluating the growth rate of thrombus under different pathological or pharmacological conditions.