脑络欣通及其拆方对脑缺血再灌注大鼠海马CA1区Fas、Fas L蛋白及皮质Fas mRNA表达影响的实验研究

目的： 探讨脑络欣通及其拆方对局灶性脑缺血再灌注大鼠海马CA1区Fas、FasL蛋白及皮质Fas mRNA表达的影响。方法： 采用线栓法大鼠大脑中动脉阻塞复制局部脑缺血再灌注模型,缺血2 h分别再灌注 1 d、3 d、7 d，随机分为假手术组、模型组、益气药组、活血药组和脑络欣通组。应用免疫组化和原位杂交法,观察缺血侧海马CA1区Fas、FasL蛋白及额顶叶皮质Fas mRNA表达。结果： 局灶性脑缺血再灌注后,缺血侧海马CA1区Fas、FasL蛋白表达平均吸光度值比假手术组增强(P＜0.01），额顶叶皮质Fas mRNA表达平均吸光度和阳性面积值强于假手术组(P＜0.01），脑络欣通组Fas、FasL蛋白和Fas mRNA表达各时段均显著低于模型组(P＜0.05或P＜0.01)；其拆方益气药、活血药组于3 d、7 d显著低于模型组(P＜0.05或P＜0.01)；脑络欣通组于3 d或/和 7 d 显著低于其拆方益气药、活血药组(P＜0.05或P＜0.01)。结论： 脑络欣通及其拆方益气药、活血药可通过抑制海马CA1区Fas、FasL蛋白和Fas mRNA表达，抗局部脑缺血再灌注神经细胞的凋亡，减轻病理性损伤。

Effect of naoluo xintong on expression of Fas, Fas L in hippocampus CA1 area and Fas mRNA in the cortex of frontal or parietal lobe after local cerebral ischemia/reperfusion in MCAO rats

AIM: To investigate the effets of naoluo xintong on the expression of Fas,FasL protein in hippocampus CA1 area and Fas mRNA in the cortex of frontal or parietal lobe after local cerebral ischemia/reperfusion in MCAO rats.METHODS: The local cerebral ischemia /reperfusion model was established by intraluminal thread occlusion of the middle cerebral arteries(MCAO),the middle cerebral arteries of rats were occluded for 2 hours and reperfused for 1,3 and 7 days.The animals were divided into pseudo surgery group(sham group),model group,Yiqi group,Huoxue group and naoluo xintong group.Using the techniques of immuno-histochemical staining and in situ hybridization,the expression of Fas and FasL was observed in hippocampus CA1 area,the expression of Fas mRNA was also observed in the cortex of frontal and parietal lobe.RESULTS: A value of Fas and FasL protein expression or A value and positive unit of Fas mRNA expression in control group were higher than those in sham in hippocampus CA1 area,the cortex of frontal or parietal lobe after local cerebral ischemia/reperfusion in MCAO rats(P<0.01).A value and/or positive unit of their expression in naoluo xintong group were lower than those in control group(P<0.05 or P<0.01).A value and/or positive unit of their expression in Yiqi and Huoxue groups were higher than those in naoluo xintong group for 3 and/or 7 days(P<0.05 or P<0.01).CONCLUSION: naoluo xintong could resist neuron apoptosis,alleviate pathologic injury after local cerebral ischemia/reperfusion in MCAO rats by inhibiting the expression of Fas,FasL protein and Fas mRNA.