耐亚胺培南鲍曼不动杆菌同源性及耐药机制研究 |
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引用本文: | 卢红梅.耐亚胺培南鲍曼不动杆菌同源性及耐药机制研究[J].中国现代医生,2012,50(18):56-57,60. |
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作者姓名: | 卢红梅 |
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作者单位: | 浙江省嘉兴市妇幼保健院检验科,浙江嘉兴,314000 |
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摘 要: | 目的调查耐亚胺培南鲍曼不动杆菌在各科室的流行特点,研究其耐药机制。方法收集不同科室分离的耐药菌54株,采用Vitek-2Compact进行药敏实验;采用PFGE分析同源性;PCR及DNA测序检测耐药基因型。结果PFGEA型占77.8%,B型占18.9%。54株菌都携带OXA-66基因,53株菌携带OXA-23基因,41株菌携带16SrRNA甲基化酶annA基因。结论临床科室存在A型耐药菌的克隆播散。产OXA-23型碳青霉烯酶基因及其上游插入序列ISAbaI是其最主要的耐药机制。
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关 键 词: | 鲍曼不动杆菌 耐药性 碳青霉烯酶 16S rRNA甲基化酶 脉冲场凝胶电泳 |
Study on the resisitance mechanism and homology of imipenem-resistant acinetobacter baumannii |
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Authors: | LU Hongmei |
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Institution: | LU Hongmei Department of Laboratory,Maternal and Child Health Care Center of Jiaxing City,Zhejiang Province,Jiaxing 314000,China |
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Abstract: | Objective To investigate the resistant mechanism to carbapenems and molecular epidemiological characteristics of imipenem-resistant acinetobacter baumannii from clinical isolates.Methods A total of 54 imipenem-resistant acinetobacter baumannii isolates were collected from different clinical departments.The Vitek-2 Compact identify bacteria,susceptibility testing and determination of MIC values;Pulsed-field gel electrophoresis was performed to analyze homology of these isolates;PCR and DNA sequencing were used to detected several carbapenemase genes and 16S rRNA methylase genes.Results Genotype A distributed in various departments as a majorwas epidemic clone.All isolates contained the blaoxa-66 carbapenemase gene,53 isolates contained blaoxa-23 and ISAbaⅠuspstream of the gene,16S rRNA methylase gene armA was identified in 41 IRABs isolates.Conclusion There has been clonal A spread of IRAB strain among patients in the hospital.Most of imipenem-resistant acinetobacter baumannii contained blaoxa-23 and ISAbaⅠuspstream of the gene. |
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Keywords: | Acinetobacter baumannii Resistance Carbapenems 16S rRNA methylase Pulse field gel electrophoresis |
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