应用共聚焦激光扫描显微镜对白癜风成像的观察与研究

目的应用共聚焦激光扫描显微镜观察白癜风皮损及周边皮肤图像特征。方法对46例白癜风患者69处皮损及周边皮肤进行共聚焦激光扫描显微镜检测,获得白癜风皮损特征性图像。结果皮损周边皮肤图像显示棘细胞层下部和基底层的角质细胞因含有黑素呈明亮的"圆形"或"椭圆形"色素环,皮损处图像显示色素环的数量及亮度呈不同程度减少或消失。结论共聚焦激光扫描显微镜是一项新的无创性皮肤影像技术,具有在体、实时、动态三维计算机断层成像的特点,可以直接观察到白癜风患者皮损处及周边角化细胞内黑素的形态变化,可为临床提供客观的评估依据。

The application of confocal laser scanning microscopy to observe and research the vitiligo image

Objective To apply the confocal laser scanning microscopy(CLSM) into observe the characteristic images of the lesion and perilesional skin of vitiligo.Methods The 69 lesions and perilesional skin of 46 vitiligo cases were examined by CLSM and the characteristic images of the lesion and perilesion of vitiligo were obtained.Results The CLSM images of perilesional skin showed bright melanin ring which appeared of roundness or ellipse at the level of keratotic call spinosum and basal stratum.But the density and brightness of melanin ring had been reduced and disappear in lesion of vitiligo for different degree.Conclusion The CLSM is a new technique of noninvasive skin imaging.The characterization of scanning imaging has in vivo,real time and dynamic feature.The technique has been applying into observe directly structure of melanin of the lesion and perilesion of vitiligo and it also can provide objective assessment for clinic.