离辐射对小鼠EL-4淋巴瘤细胞caspase-3蛋白表达的影响

目的：观察电离辐射对caspase-3蛋白表达的影响。方法：采用体外传代培养的小鼠EL-4淋巴瘤细胞为实验材料；采用单克隆抗体免疫荧光标记，FACScan流式细胞仪(FCM)检测蛋白表达的变化；采用PI荧光标记及FCM测定细胞凋亡的变化；量效实验, 观察0.5、1.0、2.0、4.0及6.0 Gy X射线照射后24 h EL-4细胞caspase-3蛋白表达及细胞凋亡的变化；时效实验, 观察4.0 Gy照射后2、4、8、12、24、48及72 h EL-4细胞caspase-3蛋白表达的变化。结果：量效实验表明，0.5、2.0、4.0及6.0 Gy X射线照射后24 h EL-4细胞中caspase-3蛋白表达均明显增高，与假照射对照组比较，差异具有显著性 (P<0.05或P<0.01)。0.5、1.0及4.0 Gy 照射后24 h EL-4细胞凋亡明显增高，与假照射对照组比较，差异具有显著性 (P<0.05或P<0.01)。 时效实验表明，4.0 Gy照射后8、12及24 h EL-4细胞中caspase-3蛋白表达均明显增高, 与同时间点假照射对照组比较，差异具有显著性 (P<0.01或P<0.001)。结论：电离辐射可诱导EL-4细胞caspase-3蛋白表达增高。同时诱导EL-4细胞凋亡。

Effect of ionizing radiation on caspase-3 protein expression in EL-4 cells in mice

Objective To investigate the effect of ionizing radiation on caspase-3 protein expression of EL-4 cells.Methods Mouse lymphoma EL-4 cell line was used.Flow cytometry(FCM) was used to examine caspase-3 protein expression and apoptosis.For dose-effect experiment,caspase-3 protein expression and apoptosis were measured(24 h) after X-irradiation with doses of 0.5,1.0,2.0,4.0 and 6.0 Gy.For time course experiment,caspase-3 protein expression was measured at 2,4,8,12,24 and 72 h after 4.0 Gy X-irradiation.Results In dose-effect experiment,it was demonstrated that the caspase-3 protein expression of EL-4 cells was increased significantly 24 h after X-irradiation with the doses of 0.5,2.0,4.0 and 6.0 Gy compared with sham-irrad(P<0.05 or P<0.01).It was also showed that apoptosis of EL-4 cells was induced significantly 24 h after X-irradiation with the doses of 0.5,1.0 and 4.0 Gy compared with sham-irrad(P<0.05 or P<0.01).In time course experiment,the result showed that the caspase-3 protein expression of EL-4 cells was increased significantly 8,12 and 24 h after 4.0 Gy (X-irradiation) compared with sham-irrad(P<0.01 or P<0.001).Conclusion Caspase-3 protein expression and apoptosis in EL-4 cells could be induced by X-rays.