Expansion and immunological study of human tumor infiltrating gamma/delta T lymphocytes in vitro. |
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| Authors: | S Yu W He J Chen F Zhang D Ba |
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| Institution: | Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, People's Republic of China. |
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| Abstract: | Goffa/delta T cells have stimulated a lot of interest because of their unique features in antigen recognition and cytotoxicities to many autologous and/or allogeneic tumor cells. We have developed a novel method to selectively expand larger amounts of human tumor-infiltrating gamma/delta T lymphocytes (gamma/delta TILs) ex vivo by immobilized pan- anti-TCRgamma/delta monoclonal antibody in the presence of exogenous IL-2. The expanded gamma/delta TILs mainly expressed CD45RO and HLA-DR molecules and did not express CD4. CD8+ gamma/delta TILs accounted for 19% of gamma/delta TILs. The expression of CD25 molecule on expanded gamma/delta T cells was inducible and downregulated following a time course. The Vdelta1 and Vdelta2 subsets amount to 37 and 58%, respectively. The expanded gamma/delta TILs show an IL-2-dependent proliferation, MHC class I-unrestricted and TCRgamma/delta-related cytotoxicities to two MHC class I+ and two MHC class I+ allogeneic tumor cell lines in vitro. |
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