人乳头状瘤病毒和p53基因及H-ras基因在喉癌细胞中的表达

目的：研究喉癌细胞p53基因、H－ras基因突变及其与人乳头瘤病毒（HPV）感染的关系，探讨其在喉癌发生发展中可能起的作用。方法：采用分子生物学技术，包括聚合酶链反应、单链构型多态性分析、限制性内切酶长度多态性分析，及DNA序列分析等方法，对28例喉癌进行人乳头状瘤病毒、p53基因及H－ras基因检测。结果：28例喉癌中有50％在p53基因的第7－8外显子有突变，其中4例行DNA序列分析表明均有碱基替换、插入或丢失；而H－ras基因第12密码子的突变率仅3．57％；高危型HPV（HPV－16或－18）感染在喉癌细胞中有一定的特异性，且与p53基因突变有一定的相关性。结论：在喉癌的发生发展过程中高危型HPV感染和p53基因突变起着重要的作用，且两者之间可能存在某种内在的联系。

Express of human papillomavirus, p53 and H-ras gene in laryngeal cancer

Objective: To study the abnormality of p53 and H ras gene in laryngeal cancer cells and their relation to the infection of human papillomavirus (HPV) subtypes. Methods: The HPV subtypes, p53 and H ras gene were examined in 28 cases of laryngeal cancer by polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing. Results: p53 gene mutation existed in 50% cases of laryngeal cancer and DNA sequencing showed base substitution, insertion or deletion in 4 cases; The positive rate of the H ras oncogene mutation was only 3.57%; All high risk HPV infections(HPV 16 or 18) were distinctly seen in the cancer cases, especially in cases with p53 mutation. Conclusion: The high risk HPV subtype infection and p53 gene abnormality may play an important role in laryngeal cancer progression and these two factors may be correlated to each other.