釉基质蛋白对人牙周膜细胞群骨钙素的影响

目的研究人牙周膜细胞群(human periodontal ligament cell populations,hPDLPs)在釉基质蛋白(em-dogain,EMD)诱导下骨钙素(osteocalcin,OCN)表达的改变。方法组织块法分离培养人牙周膜细胞,实验组用含100 mg/L EMD的培养液诱导6 d,对照组不经EMD诱导培养。免疫细胞化学和实时定量聚合酶链反应(polymer-ase chain reaction,PCR)检测成牙骨质细胞矿化相关蛋白OCN的表达。结果免疫细胞化学结果显示,实验组和对照组hPDLPs细胞胞浆均可见黄色或棕黄色颗粒,OCN表达呈阳性;实验组hPDLPs的OCN检测平均光密度值为0.172 43±0.014 85,对照组为0.167 01±0.017 03,组间差异无统计学意义(t=0.757,P=0.459);实时定量PCR检测结果显示,实验组骨钙素相对表达量是对照组的1.13倍。结论 EMD对hPDLPs的OCN表达无明显影响。

Effects of emdogain on the osteocalcin in human periodontal ligament cells

Objective To understand the expression changes of osteocalein in human periodontal ligament cells under induction of emdogain （EMD）. Methods Cultured human periodontal ligament cell populations （hPDLPs） were ex posed to the conditioned culture media containing 100 mg/L EMD for six days. Expression of osteocalcin was detected by imnmnohistochemist17 and real time polymerase chain reaction. Results hnmunohistochemistry results showed that yellow or brown granules in the cell cytoplasm were visited in the experimental group and the control group hPDLPs; the average optical density value of OCN in the experimental group hPDLPs was 0. 172 43 ±0. 014 85, and that was O. 167 01 ± 0. 017 03 in the control group hPDLPs, there was not statistically significant between the two groups （t =0. 757, P = 0. 459）. Real time polymerase chain reaction showed that the relative expression of osteocalcin is 1.13 times in the ex- periment group, compared to the control group. Conclusion The expression of osteocalcin in human periodontal liga ment cells induced was not affected by EMD.