中波紫外线对实验性矽肺大鼠氧化损伤影响

目的探讨中波紫外线照射对实验性矽肺大鼠氧化损伤的影响。方法健康成年SD雄性大鼠32只,随机分为4组,通过一次性非暴露式气管注入二氧化硅粉尘混悬液及施加中波紫外线照射后,采用比色法测定大鼠组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(HYP)和血清中铜兰蛋白(CP);测量大鼠体重增长量、脏器湿重。结果(1)注入二氧化硅粉尘后大鼠肺组织匀浆中SOD活性明显降低(P<0.01),肺匀浆中MDA含量显著升高(P<0.01);肺组织匀浆中HYP含量和血清中CP活性显著升高(P<0.05)。(2)照射中波紫外线后大鼠肺组织匀浆中SOD活性降低(P<0.01);MDA、HYP和CP在照射中波紫外线后均无变化(P>0.05)。(3)中波紫外线和二氧化硅粉尘对大鼠肺组织匀浆中SOD、HYP和血清中CP存在交互作用(P<0.01);中波紫外线和二氧化硅粉尘对大鼠肺组织匀浆中MDA存在交互作用(P<0.05)。结论二氧化硅粉尘可致大鼠肺组织氧化损伤,导致肺组织纤维化;而中波紫外线的照射可以降低矽肺模型大鼠肺组织的氧化损伤程度,减轻模型鼠肺组织纤维化程度。

Influence of UVB Irradiation on oxidative damage of experimental silicosis rat

ObjectiveTo explore the influence of UVB irradiation on oxidation damage of experimental silicosis rat. MethodsThirty two SD rats were randomly divided in to four groups.Rats in the experiment were injected with SiO₂ and irradiated with UVB.The rats were killed and their blood and lung tissue were collected.The contents of SOD,MDA and HYP in 10% homogenate of lung tisue and the contents of CP in serum were detected by colourometry.Body weight gain and organ weights were determined.ResultsAfter in jecting SiO₂ ,the activities of SOD in lung tissues homogenate were decreased(P<0.01)and the contents of HYP of lung were increased(P<0.01).After injecting SiO₂,the serum CP activities and the contents of HYP were higher than non injected rats(P<0.05).After irradiated with UVB,the activities of SOD in lung tissues homogenate were decrea sed(P<0.01).The changes of MDA ,HYP and CP were not statistically significant(P>0.05).After the exposure of UVB and silica particles,SOD and HYP in lung tissue homogenate and CP in serum showed interaction with the exposure(P<0.01).MDA in lung tissues homogenate showed interactions with the treatment(P<0.05).ConclusionSiO₂ has oxidation effect on pumlonary tissue of experimental silicosis rats and can cause pumlonary fibrosis.But UVB exposure could decrease the oxidation effect and mitigate pumlonary fibrosis.