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| 中药蒲公英的质量标准研究 | |
| 引用本文： | 凌云,范国强,肖樾,郑俊华. 中药蒲公英的质量标准研究[J]. 中草药, 1999, 30(12): 897-899 |
| 作者姓名： | 凌云 范国强 肖樾 郑俊华 |
| 作者单位： | 1. 海军总医院药剂科,北京,100037 2. 北京市中药科学研究所 3. 北京医产大学药学院 |
| 摘 要： | 用ＨＰＬＣ法测定蒲公英中咖啡酸和绿原酸的含量。采用 ＨＰ Ｈｙｐｅｒｓｉｌ Ｃ_１８色谱柱，２００ ｍｍｘ ２． １ｍｍ，５μｍ，流动相：甲醇－磷酸盐缓冲溶液（ｐＨ＝４． ２） ２３： ７７，检测波长：３２３ ｎｍ，柱温：４０℃，流速：１．０ ｍＬ／ｍｉｎ。该法咖啡酸平均回收率 ９９．８０％，ＲＳＤ ２．１４％；绿原酸平均回收率９９．５４％，ＲＳＤ ２．６９％。本法稳定可靠，为药典建立蒲公英药材的质量标准提供了依据。 |
| 关 键 词： | 蒲公英 咖啡酸 绿原酸 质量标准 高效液相色谱 |
| 收稿时间： | 1999-08-17 |
| 修稿时间： | 1999-08-17 |
Studies on Quality Standard of Dandelions (Taraxacum Weber) |
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| Ling Yun,Fan Guo-qiang,Xiao Yue and Zhen Jun-hua. Studies on Quality Standard of Dandelions (Taraxacum Weber)[J]. Chinese Traditional and Herbal Drugs, 1999, 30(12): 897-899 | |
| Authors: | Ling Yun Fan Guo-qiang Xiao Yue Zhen Jun-hua |
| Affiliation: | Department of Pharmacy, Navy General Hospital, Beijing 100037 |
| Abstract: | The establishment of a quality standard by HPLC for caffeic acid and chlorogenic acid in dandelions, Tarazacum Weber, was reported. The sample was extracted with methanol under ultrasonic management for 30 min. Chromatographic conditions:YMS-ODS C, e column, 200 mm × 2.1 mm, 5 μm, a mobile phase consisted of methanol and potassium phosphate (23:77, pH 4.2), a flow rate of 1. 0 mL/min;a detecting wavelength at 323 nm and the column temperature at 400C.The mean recovery rate of caffeic acid was 99. 80%, RSD 2.14 % . The mean recovery rate of chlorogenic acid was 99. 54%, RSD 2. 69 %. The method was very stable, accurate, and can be used as a quality standard for dandelions. |
| Keywords: | Taraxacum Weber HPLC caffeic acid chlorogenic acid |
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