Novel therapeutic biosensor for indinavir—A protease inhibitor antiretroviral drug |
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Authors: | A. Ignaszak N. HendricksT. Waryo E. SongaN. Jahed R. NgeceA. Al-Ahmed B. KgarebeP. Baker E.I. Iwuoha |
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Affiliation: | SensorLab, Department of Chemistry, University of Western Cape, Bellville, Cape Town 7535, South Africa |
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Abstract: | An amperometric drug metabolism biosensor consisting of cytochrome P450-3A4 (CYP3A4) encapsulated in a didodecyldimethylammonium bromide (DDAB) vesicular system on a Pt disk electrode was developed for the determination of indinavir, a protease inhibitor antiretroviral drug. Cyclic, square wave and pulse voltammetric responses of the bioelectrode showed quasi-reversible electrochemistry of the Fe3+/Fe2+ redox species of the heme thiolate CYP3A4 enzyme under aerobic and anaerobic conditions. The biosensor exhibited excellent response to indinavir with a detection limit and response time of 6.158 × 10−2 mg L−1, and 40 s, respectively. The detection limit is well below the plasma concentration of indinavir (8 h after intake) which range from 0.13 to 8.6 mg L−1. |
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Keywords: | Drug metabolism biosensor CYP3A4 Indinavir Amperometric biosensor Bioelectrode |
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