白藜芦醇抑制U251人胶质瘤细胞增殖及其相关机制的探讨

目的 探讨白藜芦醇（Res）对U251胶质瘤细胞增殖和侵袭的抑制作用及其机制。方法 将不同浓度Res作用于U251胶质瘤细胞系，相差显微镜下观察其形态的变化，MTT法检测其对细胞生长增殖的抑制作用，流式细胞术（FCM）检测其对细胞周期的影响，用蛋白印迹（Western blot）检测Res处理前后U251细胞表皮生长因子受体（EGFR）、p53、p21、CDK4、CyclinD1、Bcl-2及caspase-3的表达，免疫组化分析MMP9、NFKB、P-AKT及AKT2的表达。结果 U251细胞经Res处理后，细胞形态发生一定变化，U251胶质瘤细胞的增殖被抑制，呈浓度和时间依赖性，细胞周期被阻滞在S期，Western blot检测显示EGFR、CyclinD1、CDK4、Bcl-2的表达降低，p21、p53、caspase-3蛋白表达升高，免疫组化检测显示MMP9，NFKB、P-AKT及AKT2的表达降低。结论 Res有可能通过调节EGFR及p53信号通路而抑制U251胶质瘤细胞的增殖和侵袭，诱导肿瘤细胞凋亡。

Suppressive effect of resveratrol on the proliferation of human U251 glioma cells and its mechanism

Objective To investigate the suppressive effect of resveratrol on proliferation of U251 human glioma cells and its mechanism. Methods U251 glioma cells were treated with resveratrol at various concentrations, the morphology of cells was detected by phase contrast microscopy. MTT assay was used to determine the inhibitory rate of cell growth, FCM to detect cell cycle before and after the resveratrol treatment, and Western blotting to examine the expression of EGFR, p53, p21, CyclinD1, CDK4, Bcl-2andcaspase-3. The expressionsofMMP9, NFKB, p-AKTandAKT2were analysed by immunohistochemistry. Results After treatment with resveratrol, the cells occurred great morpgological and many cells died. MTT assay showed the growth of U251 cells was inhibited in a dose-and time-dependent manner. Cell cycle was blocked in S phase, Western blotting showed that resveratrol downregulated the expression of EGFR, CyclinD1, CDK4, Bcl-2, but upregulated p53, p21 and caspase-3. Immunohistochemical staining displayed the decreased expression of MMP9, NFKB, p-AKT and AKT2. Conclusion It is possible that resveratrol inhibits the cell proliferation and invasion via modulation of EGFR and p53 signaling pathway.