快速原位杂交技术在感染性病原体诊断中的应用

快速原位杂交技术是一种近年才发展起来的检测常规病理标本中有无感染原存在的非常有效的工具,与现在普遍采用的原位杂交技术相比具有更强的核酸定位能力.作者采用该项新技术,用非同位素标记的寡聚核苷酸探针以及毛细虹吸技术完成常规福尔马林固定、石蜡包埋病理标本中感染原的检测.结果显示,使用这种快速原位杂交技术能在1小时内完成鼻咽癌组织和非鼻咽癌鼻咽部组织中的爱泼斯坦-巴尔病毒和宫颈组织中的人类乳头状瘤病毒的检测,且染色清晰,定位准确,效果满意,可广泛应用于临床病理实验室的日常检测工作以及感染性病原体相关疾病的研究.

Application of Less-than-one-hour in situ Hybridization to Diagnosis of I nfectious Agents

Less-than-one-hour in situ hybridization manual method for immunocytochemical analyses of formalin-fixed, paraffin embedded tissue sections was used to detect infectious agents. The method employs capillary action to sequentially apply, incubate and remove liquid reagents from apposite pairs of glass microscope slides and allows for simultaneous immunocytochemical analyses. We used this method to detect EB virus in nasopharyngeal tissues and human papilloma virus in cervical tissues. This rapid and sensitive procedure represents a significant improvement for clinical immunocytochemical and research laboratories alike.