高效液相色谱法测定止嗽青果合剂中麻黄碱含量

目的：建立止嗽青果合剂中盐酸麻黄碱含量测定方法。方法：采用Kromasil—C18（4．6mm×250mm，5μm）色谱柱，以乙腈-0．1％磷酸溶液（含0．1％三乙胺）=3：97为流动相，流速为1．0mL·min^-1，检测波长为207nm。结果：麻黄碱进样量在0．406～1．596μg范围内具有良好的线性关系，回归方程为：Y=738．3x＋0．0347（r=0．9999，n=5）；平均加样回收率为98．9％，RSD=0．8％；止嗽青果合剂中麻黄碱的含量应不少于0．260mg·mL^-1。结论：HPLC法测定止嗽青果合剂中麻黄碱含量．操作简便．结果准确．可用于止嗽青果合剂的质号控制．

Determination of Ephedrine Hydrochloride in Zhisouqingguo Mixture by HPLC

Objective： To establish a HPLC method for the content determination of ephedrine hydrochloride in zhisouqingguo mixture. Methods： Kromasil-C18 column （4.6 mm× 250 mm, 5 μm） was used for separation. The mobile phase was acetonitrile- 0.1% phosphoric acid （0.1% triethylamine） （3 ： 97）. The flow rate was 1.0 mL · min^-1, and the detection wavelength was set at 207 nm. Results： The linear range of ephedrine hydrochloride was 0.406 - 1.596 μg and the regression equation： y = 738.3x ＋ 0.034 7 （r = 0.999 9, n = 5）. The average recovery rate was 98.9% with RSD = 0.8%. The content of ephedrine hydrochloride in zhisouqingguo mixture should not be less than 0.260 mg · mL^-1. Conclusion： This method for the content determination of ephedrine hydroehloride is simple and accurate, which can be used for the quality control ofzhisouqingguo mixture.