反相高效液相色谱法快速测定日本豆腐中的柠檬黄

目的建立了一种前处理简单、灵敏度高、重现性好的日本豆腐中柠檬黄的检测方法。方法采用反相高效液相色谱法,AgilentHC-C18柱(250mm×4.6mm,5μm)液相色谱柱分离,乙酸铵缓冲溶液(0.02mol/L)-甲醇为流动相进行梯度洗脱,二级管阵列检测器,检测波长448nm。结果测定结果具有良好的线性(r=0.9996),保留时间的RSD为0.18%(n=6),峰面积的RSD为0.85%(n=6),样品加标回收率为87.4%～95.5%,方法检出限为0.002g/kg。结论该方法具有操作简便、快速,重复性好、灵敏度高等优点,可用于快速测定日本豆腐中的柠檬黄。

Rapid Detection the Tartrazine in Japanese Tofu by RP-HPLC

Objective To establish a simple pre-treatment,high sensitivity and nice reappearance method for detecting the tartrazine in Japanese Tofu.Method Using RP HPLC,separation the compounds based on Agilent HC-C18（250mmⅹ4.6mm,5μm）and mobile phase of ammonium acetate buffer solution（pH4.5）-methanol by gradient elution,the respond was recorded by DAD.The detection wavelength was 448nm.Results The calibration curve of tartrazine showed good linear（r=0.9 996）and well repeated.The detection limit was 0.002g/kg,relative standard deviation（RSD）was 0.18% for retention time and 0.85% for peak area.The recoveries were in the range of 7.4% to 95.5%.Conclusions The method is simple,sensitive,accurate and rapid,and suitable for determination the tartrazine in Japanese Tofu.