姜黄素对糖尿病大鼠肾组织c-fos及c-jun基因表达上调的抑制作用

目的探讨姜黄素对糖尿病大鼠肾组织c-fos及c-jun基因表达上调的影响。方法大鼠随机分为正常对照组(A组)、糖尿病组(B组)及姜黄素治疗组(C组),采用腹腔单剂量注射链脲佐菌素(streptozo-tocin,STZ)65mg/kg建立糖尿病大鼠模型。治疗组给予姜黄素30mg/kg-1.d-1腹腔注射。实验第4周各组分别宰杀5只大鼠,取部分右肾皮质,采用RT-PCR及免疫组织化学方法分别检测姜黄素对糖尿病大鼠肾组织c-fos mRNA、c-jun mRNA及其蛋白表达上调的影响,并观察肾组织的病理学变化。结果糖尿病大鼠肾组织c-fos mRNA、c-jun mRNA及其蛋白表达明显上调,姜黄素可显著抑制上述指标表达上调,并减轻糖尿病大鼠肾脏病变。结论姜黄素通过抑制糖尿病大鼠肾组织c-fos及c-jun基因表达上调而减轻糖尿病大鼠肾脏损伤。

Inhibition of Curcumin on up Regulation c-fos and c-jun mRNA in Kidney of Diabetic Rats

Objective To study the effects of curcumin on up-regulate c-fos and c-jun mRNA in the kidney of diabetic rats.Method Rats were randomly divided into normal control rats（group A）,diabetic rats group（group B）,and diabetes treated with curcumin group（group C）.DM rats were induced by STZ（65mg/kg）,treatment group was given intraperitoneal injection of curcumin 30mg·kg-1·d-1.Five rats were killed in each group at the 4th week,and took the renal cortex of right kidney,used RT-PCR and immunohistochemistry to detect the expressions of c-fos and c-jun mRNA and protein level,respectively,and observed the pathological changes of kidney.Results The expression of c-fos and c-jun mRNA and protein levels in the kidney of diabetic rats was significantly up-regulate after 4 weeks,but those targets were reversed in diabetic rats treated by curcumin.Accordingly,renal pathologic changes were significantly improved.Conclusions Curcumin can decrease the damage of diabetic rats＇ kidney through inhibiting the up regulation of c-fos and c-jun mRNA and protein levels in renal cortices.