补肾壮骨颗粒含药血清对骨质疏松大鼠骨髓间充质干细胞增殖的影响

目的：探讨补肾壮骨颗粒含药血清对去卵巢骨质疏松大鼠骨髓间充质干细胞（ MSCs）体外增殖的影响。方法：选用10只6月龄SD大鼠去卵巢制作骨质疏松模型，对骨质疏松骨髓间充质干细胞进行分离和体外培养，传代后的 MSCs分为OP组、OP＋补肾壮骨颗粒含药血清组和OP＋空白血清组，倒置显微镜观察各组MSCs的生长情况，进行形态学观察，并用四甲基偶氮唑蓝（ MTT）法测定生长曲线。结果：OP＋含药血清组，MSCs呈典型的集落生长，细胞呈长梭形，突起细长且多，细胞增殖较旺盛，细胞数量不断增加，随着传代次数的增多，细胞增殖速度减慢。OP组及OP＋空白血清组MSCs接种后胞体立体感下降，体积较大，细胞偏圆形，突起收缩，增殖速度较慢，细胞数量减少；随着传代次数的增多，细胞增殖速度显著减慢。OP＋补肾壮骨颗粒含药血清组骨髓间充质干细胞增殖能力明显提高，生长曲线有显著性差异。结论：补肾壮骨颗粒含药血清能促进去卵巢骨质疏松大鼠MSCs的增殖。

Effects of Serum Containing Bushen Zhuanggu Granules on Proliferation of Mesenchymal Stem Cells of Osteoporotic Rats

Objective：To investigate the effect of serum containing Bushen Zhuanggu granules in-vitro proliferation of mesenchymal stem cells（ MSCs）of osteoporotic（ OP）rats. Methods：10 cases osteoporotic animal models were established in 6 months old SD rats by ovari-ectomy. MSCs were isolated from the OP rats and cultured in vitro. Cultivated MSCs were divided into 3 groups：OP group,OP＋serum containing bushen zhuanggu granules group and OP＋ blank serum group. The morphological difference of the MSCs in 3 groups was ob-served under inverted phase contrast microscope. Optical absorption value of MSCs was stained by methyl thiazolyl tetrazolium（ MTT）. The growth curve was measured. Result：In OP＋serum containing bushen zhuanggu granules group,MSC was typical colony growth. The cell was long fusiform shape,which ecphyma of cell was slender and more. The cell proliferation was prosperity,population was increas-ing. But the rate of cell proliferation was to step down,when passage of cell was increasing. In OP group and OP＋blank serum group, MSC body was comparatively large,meta-round shape. Ecphyma of cell shrink,the rate of proliferation was to step down,population was decreased. When passage of cell was increasing,step down of cell proliferation was significant. In OP ＋ serum containing Bushen Zhuanggu granules group proliferative activity of MSCs was significantly promoted,the growth curve was significant difference in compar-ison with the control group. Conclusion：Serum containing Bushen Zhuanggu granules can enhance the proliferation of MSCs in OP rats.