| 高效液相色谱法测定抗眩颗粒中葛根素的含量 |
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| 引用本文： | 吕宏迪,范莉莉,李文俊,张正臣. 高效液相色谱法测定抗眩颗粒中葛根素的含量[J]. 河南中医药学刊, 2014, 0(10): 1484-1486 |
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| 作者姓名： | 吕宏迪 范莉莉 李文俊 张正臣 |
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| 作者单位： | 中国人民解放军第371中心医院,河南新乡453000 |
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| 基金项目： | 河南省科学技术厅2009年科技发展计划（编号：092102310202） |
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| 摘 要： | 目的：建立抗眩颗粒中葛根素的含量测定方法。方法：以甲醇-0.1磷酸溶液（25：75）为流动相;检测波长为250 nm。理论板数按葛根素峰计算应不低于1 000。结果：葛根素浓度在12～120 mg·L^-1范围内,峰面积与浓度线性关系良好,平均回收率为99.63%。结论：高效液相色谱法测定抗眩颗粒中葛根素的含量,操作简单、重复性好,可作为抗眩颗粒的质量控制方法。
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| 关 键 词： | 抗眩颗粒 葛根素 高效液相色谱法 含量测定 |
Determination the Content of Puerarin in Kang Xuan Particles by HPLC Method |
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| Lü Hongdi,Fan Lili,Li Wenjun,Zhang Zhengchen. Determination the Content of Puerarin in Kang Xuan Particles by HPLC Method[J]. Journal of Henan college of Traditional Chinese Medicine, 2014, 0(10): 1484-1486 |
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| Authors: | Lü Hongdi Fan Lili Li Wenjun Zhang Zhengchen |
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| Affiliation: | (The 371st Central Hospital of the Chinese People＇s Liberation Army,Xinxiang Henan China 453000) |
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| Abstract: | Objective： To establish method for the determination of puerarin in Kang Xuan Particles. Methods： Methanol with 0. 1% phosphoric acid solution（ 25∶75） as mobile phase; the detection wavelength was 250 nm. Number of theoretical plates by puerarin peak calculation should not be less than 1000. Results： Puerarin concentration in the range of 12- 120 μg ·mL^-1 range,the peak area and the concentration of a good linear relationship,the average rate of recovery is 99. 63%. Conclusion： HPLC method for the determination of Resistance to dizzy particles of puerarin content,simple operation,good reproducibility,can be used as the quality control method of Resistance to dizzy particles. |
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| Keywords: | kang xuan particles puerarin HPLC content determination |
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