芍药苷对抑郁大鼠海马组织形态及BDNF水平的影响

目的?在大鼠强迫游泳急性抑郁动物模型上观察芍药苷的抗抑郁作用及可能机制。方法?SD大鼠随机分为对照组、模型组、芍药苷组及氟西汀组，每组各10只。模型组、芍药苷组及氟西汀组强迫游泳15min建立急性抑郁模型，给药组分别灌胃芍药苷10mg/kg，氟西汀20mg/kg，24h给药3次，对照组给等量生理盐水。观察行为学开场实验；ELISA检测血清BDNF、TrkB水平及海马组织BDNF水平；病理切片观察海马组织形态。 结果?芍药苷能够增加大鼠运动距离（P<0.01）；显著升高大鼠血清及海马组织BDNF水平（P<0.01），且对海马病理形态学有保护作用。结论?芍药苷在强迫游泳急性抑郁动物模型上有抗抑郁活性，其作用机制可能涉及中枢BDNF-TrkB受体信号转导通路。 

Effect of Paeoniflorin on Hippocamp Tissue Pathomorphology and BDNF Level of Forced Swimming Rats Model

OBJECTIVE?To observe the antidepressant effect of Paeoniflorin and the possible mechanism in acute depressed rat model of forced swimming. METHODS?SD rats were randomly divided into control group， model group， peony glycoside group and fluoxetine group（10/group）. The model group， Paeoniflorin group and Fluoxetine group were forced to swim for 15min to establish the depressed model. Treatment groups were administered paeoniflorin(10mg/kg) and fluoxetine (20mg/kg)， respectively， 3 times in 24h. Control group were treated with vehicle(saline). Observation indexes: the opening experiment of behavior， levels of BDNF in serum and hippocampus and the pathological observation of hippocampus. RESULTS?Paeoniflorin significantly increased the motility distance of rats(P<0.01) and BDNF levels in rat serum and hippocampus(P<0.01)， which displayed protective effects on hippocampus pathomorphology. CONCLUSION?Paeoniflorin shows antidepressant effect in forced swimming animal model， which probably relates to the BDNF/TrkB receptor signal transduction pathway.