| 血府逐瘀汤不同剂型数字化指纹图谱的构建 |
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| 引用本文： | 杨龙,杨帆,杨欣莹,张蕾,于卉娟,王跃飞. 血府逐瘀汤不同剂型数字化指纹图谱的构建[J]. 天津中医药, 2016, 33(9): 559-564 |
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| 作者姓名： | 杨龙 杨帆 杨欣莹 张蕾 于卉娟 王跃飞 |
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| 作者单位： | 天津中医药大学中医药研究院, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津国际生物医药联合研究院中药新药研发中心, 天津 300457,天津中医药大学中医药研究院, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津国际生物医药联合研究院中药新药研发中心, 天津 300457,天津宏仁堂药业有限公司, 天津 300385,天津中医药大学中医药研究院, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津国际生物医药联合研究院中药新药研发中心, 天津 300457,天津中医药大学中医药研究院, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津国际生物医药联合研究院中药新药研发中心, 天津 300457,天津中医药大学中医药研究院, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;天津国际生物医药联合研究院中药新药研发中心, 天津 300457 |
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| 基金项目： | 国家自然科学基金青年基金项目（81202877）。 |
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| 摘 要： | [目的]建立一种有效的超高效液相色谱(UPLC)数字化色谱指纹图谱,评价血府逐瘀汤不同剂型的质量,为血府逐瘀汤制剂(胶囊、口服液、片剂、软胶囊、汤剂)质量控制提供参考。[方法]采用超高效液相色谱仪,以色谱柱ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm),柱温50℃,体积流量0.3 m L/min,进样量2μL,检测波长为280 nm,0.1%甲酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱(0~8 min,3%~8%B;8~24 min,8%~19%B;24~35 min,19%~45%B)。[结果]构建了血府逐瘀汤多种剂型的指纹图谱。以野漆树苷为参照峰,共标定了30个共有峰,通过标准品比对确定了血府逐瘀汤不同制剂指纹图谱中的16个共有峰。采用《中药色谱指纹图谱相似度评价系统》(2004A版)评价了血府逐瘀汤不同制剂指纹图谱相似度均大于0.81。以血府逐瘀汤不同剂型共有峰的相对峰面积为特征值进行主成分分析(PCA),不同剂型样品聚类在3个不同的区域,说明血府逐瘀汤不同剂型的质量存在明显差异。[结论]该方法构建的指纹图谱简便易行,为血府逐瘀汤不同剂型的质量控制研究提供依据。
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| 关 键 词： | 血府逐瘀汤 数字化指纹图谱 主成分分析 |
| 收稿时间： | 2016-03-30 |
The establishment of UPLC-Digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction |
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| YANG Long,YANG Fan,YANG Xin-ying,ZHANG Lei,YU Hui-juan and WANG Yue-fei. The establishment of UPLC-Digitized fingerprinting of multiple dosage forms of Xuefu Zhuyu decoction[J]. Tianjin Journal of Traditional Chin Medicine, 2016, 33(9): 559-564 |
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| Authors: | YANG Long YANG Fan YANG Xin-ying ZHANG Lei YU Hui-juan WANG Yue-fei |
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| Affiliation: | Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China,Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China,Tianjin Hongrentang Pharmaceutical Co., Ltd., Tianjin 300385, China,Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China,Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China and Tianjin Modern Chinese Medicine Key Laboratory-Province and Ministry Co-established State Key Laboratory Cultivation Base, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, China |
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| Abstract: | |
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| Keywords: | Xuefu Zhuyu decoction UPLC-digitized fingerprinting principal component analysis |
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