低剂量辐射小鼠骨髓细胞凋亡率的变化

目的:探讨低剂量射线暴露小鼠骨髓细胞凋亡率的变化及意义。方法:以Co60射线照射ICR小鼠,制备不同时间段的骨髓单个核细胞的单细胞悬液,用PI/FITC-Annexin V双染法,通过流式细胞仪检测样品的凋亡率。结果:骨髓单个核细胞早期凋亡率于照射后6 h明显增高;晚期凋亡率随辐射剂量增大而增高。白细胞计数及骨髓细胞微核率于24~30 h与对照组比较差异有显著性(P<0.05,P<0.01)。结论:小鼠骨髓细胞凋亡率的变化可及时反映射线对小鼠骨髓细胞的影响。细胞凋亡率检测可能成为低剂量射线接触人群的早期监控指标。

Changes of cells apoptosis in the bone marrow induced by low doses of irradiation

Objective: To observe the changes of the apoptotic rate of mice bone marrow cells induced by low doses of irradiation and to find out early biological monitoring indexes.Methods: Mice were killed at different time after Co60 says had irradiated the whole body,the bone marrow cells were isolated,the mononuclear was prepared and suspended in PBS,then stained with PI and FITC-Annexinv and analyzed with BD FACSAria flow cytometer,subsequently.Results: It was found that only 6h after exposed to low doses of irradiation,the apoptotic rates in the early phase of mice bone marrow cells were significantly higher than that in control(P<0.05,P<0.01),and that of apoptosis in the late phase and cell death were in dose-dependent manner.The decrease of WBC in mice blood and the elevation of MN in mice bone marrow cells were at 24-30 h.Conclusion: It suggested that low doses of irradiation would increase the apoptotic rate of mice bone marrow cells,the detection of the apoptotic would be regarded as an early biological monitoring index among people who expose to low dose irradiation.