异丙酚与安氟醚或七氟醚静吸复合麻醉对氧自由基的影响

目的:观察异丙酚与安氟醚、七氟醚静吸复合麻醉对氧自由基的影响.方法:32例择期手术的全麻病人,随机分为4组(每组8例),分别给予安氟醚麻醉(A组)、七氟醚麻醉(B组)、异丙酚-安氟醚麻醉(C组)、异丙酚-七氟醚麻醉(D组).其中A、B组为对照组,C、D组为观察组.分别于麻醉前及麻醉诱导后15min、1h和2h采集静脉血测红细胞超氧化物岐化酶(SOD)、血清丙二醛(MDA).结果:麻醉后,A、B两组SOD值均逐渐下降,MDA值均逐渐升高,麻醉诱导后2hSOD、MDA值较麻醉前差异均有显著性(P<0.05),但A、B两组相比较,SOD、MDA值差异均无显著性(P>0.05);C、D两组SOD值较麻醉前均有下降趋势,但差异均无显著性(P>0.05),MDA值较麻醉前均逐渐下降,并于麻醉诱导后2 h差异均有显著性(P<0.05),但C、D两组相比较SOD、MDA值差异均无显著性(P>0.05).A与C组之间相比较及B与D组之间相比较,SOD值在麻醉诱导后2h差异均有显著性(P<0.05);MDA值在麻醉诱导后1 h及2 h差异均有显著性(P<0.05、P<0.01).结论:安氟醚和七氟醚均能产生自由基使MAD升高、SOD下降;临床麻醉剂量的异丙酚能有效地清除自由基、降低MDA.

Effect of propofol-enflurane and propofol-sevoflurane combined anaesthesia on oxygen-derived free radicals

Objective:To investigate the effect of propofol enflurane and propofol sevoflurane combined anaesthesia on oxygen derived free radicals. Methods:Thirty two adult patients scheduled for selective surgery under general anaesthesia were randomly allocated to Group A receiving enflurane, Group B receiving sevoflurane, Group C receiving propofol enflurane and Group D receiving propofol sevoflurane to maintain anaesthesia. Group A and group B were set up as control groups. The blood specimens were taken from all patients before anaesthesia and 15 min, 1 h and 2 h after induction to measure the serum concentration of SOD and MDA. Results: After anaesthesia, in Group A and group B the concentration of SOD decreased gradually and the concentration of MDA increased gradually .These changes were statistically significant ( P <0.05) 2h after induction . There was no significant differences between Group A and group B in the concentration of SOD and MDA ( P >0.05). There was also trends of SOD decrease without statistic significance ( P >0.05) in group C and group D. As MDA level decreased significantly 2 h after induction ( P <0.05) in these two groups there was no significant differences between the both groups. In comperision of Group A with group C and group B with group D the concentration of SOD decreased significantly 2 h after induction ( P <0.05) and the concentration of MAD increased significantly 1 h and 2 h after induction. ( P<0.05,P <0.01). Conclusion: Enflurane and sevoflurane also can produce oxygen derived free radicals to decrease SOD and to increase MAD; Propofol in clinical dose can eliminate oxygen derived free radicals effectively and decrease the concentration of MDA.