阿司匹林对脑缺血-再灌注大鼠脑能量代谢的影响

目的观察阿司匹林（ASA）对脑缺血一再灌注大鼠脑组织能量代谢的影响。方法取雄性sD大鼠50只，随机分为假手术组（12只）、溶剂组（26只）、ASA组（12只），根据再灌注时间将每组再分为24、72h两个亚组。线栓法制作大脑中动脉缺血2h，再灌24、72h模型。ASA组大鼠于再灌注同时灌胃给予ASA，60mg／kg（60mgASA加0．05ml无水乙醇助溶，用中性PBS液定容至10m1），1ml/100g。溶剂组大鼠灌胃给予等体积的0．5％无水乙醇PBS溶液。高效毛细管电泳分析法测定三磷酸腺苷酶（ATP）含量，无机磷法测定Na＋-K＋-ATP酶和Ca2＋-ATP酶活性，比色法测定乳酸脱氢酶及乳酸含量。结果①ASA组大鼠再灌注24、72hATP含量为（27．7±3．5）、（29．7±2．5）μml/g（湿重）；Na＋-K＋-ATP酶为（8．6±0．9）、（7．8±0．6）μmol·mg-1·h-1；Ca2＋-ATP酶为（6．1±0．8）、（6．6±0．7）μmol·mg-1·h-1，与溶剂组大鼠的（10．3±1．0）、（4．6±0．8）μmol／g（湿重），（4．5±0．7）、（5．8±0．8）及（4．5±0．4）、（2．5±0．5）μmol·mg-1·h-1比较，差异有统计学意义（P〈0．01或0．05）；与假手术组比较，除Ca2＋ATP酶含量高于假手术组（4．3±0．5）、（4．6±0．3）μmol·mg-1·h-1]外，其他指标差异无统计学意义。②ASA组再灌注24、72h乳酸含量为（0．68±0．25）、（0．62±0．15）mmol／g（蛋白）；乳酸脱氢酶活性为（9769±957）、（9677±532）U／g（蛋白）；与溶剂组大鼠的（0．42±0．12）、（0．33±0．16）mmol／g（蛋白），（4033±723）、（5902±689）U／g（蛋白）比较，差异有统计学意义（P〈0．01或0．05）；与假手术组大鼠的（0．58±0．19）、（0．61±0．23）mmol／g（蛋白），（9430±273）、（9382±375）U／g（蛋白）比较，差异无统计学意义。结论阿司匹林对脑缺血-再灌损伤24和72h大鼠脑组织均有明显的保护?

Effect of aspirin on brain energy metabolism in rats with cerebral ischemia-reperfusion injury

Abstract： Objective To observe the effect of aspirin （ASA） on brain energy metabolism in rats with focal cerebral ischemia-reperfusion injuries. Methods Fifty male SD rats were randomly allocated to sham-operation （ n = 12 ）, vehicle （ n = 26） and ASA （ n = 12 ） groups. Each group was redivided into 2 subgroups according to the time of reperfusion（ 24 h and 72 h） . The models of focal middle cerebral artery ischemia for 2 h and reperfusion for 24 and 72 h were established by suture method. The ASA group rats were given ASA, 60 mg/kg by gavage at the same time with reperfusion. The vehicle group rats were given 0.5% absolute alcohol PBS. The level of adenosine triphosphate （ATP） was determined by capillary elec- trophoresis. The Na ＋ -K ＋ -ATPase and Ca2＋ -ATPase activity were determined by inorganic phosphate as- say, and the levels of lactate dehydrogenase and lactic acid were determined by colorimetric method. Results （1）The levels of ATP after 24 h and 72 h reperfusion in the ASA group were 27. 7 ± 3. 5 and 29. 7 ± 2. 5 μmol/g wet weight ; The Na ＋ -K ＋ -ATPase were 8.6 ± O. 9 and 7.8 ± 0. 6 μmol·mg-1·h-1 ; The Ca2＋ -ATPase were 6. 1 ±0. 8 and 6.6 ±0. 7 μmol·mg-1·h-1. As compared with 10. 3 ± 1.0 and 4. 6 ± 0. 8μmol/g wet weight, 4. 5 ±0. 7 and 5. 8 ±0. 8 μmo·mg-1·h-1, and 4. 5 ＋0. 4 and 2. 5±0. 5 μmol·mg-1·h-1 in the vehicle group, the differences were statistically significant. As compare with the sham- operation group, apart from the level of Ca2＋ -ATPase was higher than that of the sham-operation group （4. 3±0. 5 and 4. 6 ±0. 3 μmol·mg-1·h-1 ）, the differences of other indexes were not statistically sig- nificant. （2）The levels of lactic acid 24 h and 72 h after reperfusion were 0. 68 ± 0. 25 and 0. 62 ± 0. 15 mmol/g protein; the lactate dehydrogenase activities were 9769 ＋ 957 and 9677 ± 532 U/g protein; As compared with 0. 33 ±0. 16 and 0. 42 ＋0. 12 mmol/g protein, and 4033 ＋723 and 5902 ＋689 U/g pro- tein in