犬急性大块肺动脉栓塞动物模型的建立

目的制作一种适合器械祛栓试验使用的犬急性大块肺栓塞动物模型。方法7只杂种犬,体外制作动物自体血栓,采用经皮股静脉穿刺置入导管,选择性插入一侧肺动脉中央分支动脉后注入制作好的自体血凝块,栓塞一侧肺动脉中央分支动脉,栓塞前后检测血气、肺动脉压及肺动脉造影。实验存活动物于12h后处死,取两侧肺组织进行病理检查。结果1只注射血栓时造成两侧肺栓塞死亡,1只在栓塞12h后肺动脉有部分再通;其余各只均成功栓塞至靶肺动脉的中央分支动脉。结论该方法制作急性大块肺栓塞的动物模型是可行和可重复的,可以为相关试验提供急性大块肺动脉栓塞动物模型。

The establishment of animal model of acute massive pulmonary embolism

Objective To find a way of establishing the model of acute massive pulmonary embolism in dog.Methods Seven dogs were selected with self-clots made outside the body transferring through a 10 F guiding catheter into the central branch of pulmonary artery via the femoral vein approach on one side and then under pressure monitor of pulmonary artery until the very branch of pulmonary artery was occluded.Blood gas and pulmonary arterial pressure were tested before and after the embolization,Pulmonary artery pressure was continuously monitored together with the examinations of angiography.The bilateral lung specimens were resected for histological examination 12 hours in average after the embolization for comparative study.Results One animal died of cardiogenic shock after clots injection;the other one presented with tachycardia and premature ventricular beat causing partial recanalization 12 h later.The others were occluded successfully in central branch of pulmonary artery and the pulmonary arterial pressure reached above 50 mmHg after occlusion.Pathologic examination showed the formation of red and mix thrombi within the vascular lumens.Conclusions This method for making acute massive pulmonary embolism animal model was reliable,feasible and reproducible,and could provide an animal model of acute massive pulmonary embolism for other correlative experiments.